Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zumft, W.G.
    Ferredoxin:nitrite oxidoreductase from Chlorella. Purification and properties (1972), Biochim. Biophys. Acta, 276, 363-375.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.7.7.1 unstable in crude extracts and in solutions below pH 7 [Chlorella] fusca

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.7.1 8-hydroxyquinoline 7% inhibition at 5 mM [Chlorella] fusca
1.7.7.1 CO
-
 [Chlorella] fusca
1.7.7.1 cyanide 90% inhibition at 1 mM [Chlorella] fusca
1.7.7.1 EDTA
-
 [Chlorella] fusca
1.7.7.1 o-phenanthroline 15% inhibition at 5 mM [Chlorella] fusca
1.7.7.1 p-chloromercuribenzoate complete inhibition at 0.1 mM [Chlorella] fusca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.7.1 Iron 2 atoms of iron probably bound in the chromophoric group [Chlorella] fusca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.7.1 63000
-
 gel filtration, SDS-PAGE [Chlorella] fusca

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.1 [Chlorella] fusca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.1
-
 [Chlorella] fusca

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.7.1 51.7
-
-
 [Chlorella] fusca

Storage Stability

EC Number Storage Stability Organism
1.7.7.1 -24°C, 30% loss of activity within 4 months [Chlorella] fusca
1.7.7.1 4°C, 30% loss of activity within 3 weeks [Chlorella] fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.1 nitrite + reduced ferredoxin other electron donor: methyl viologen [Chlorella] fusca ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin enzyme can also catalyze the reduction of hydroxylamine to ammonia, at lower rate [Chlorella] fusca ammonia + oxidized ferredoxin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.7.1 monomer 1 * 63000 SDS-PAGE + mercaptoethanol [Chlorella] fusca

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.7.1 7
-
 enzyme in crude extract is rapidly inactivated below pH 7 [Chlorella] fusca