EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.7.7.1 | CO | forms a complex with the reduced enzyme | Spinacia oleracea | |
1.7.7.1 | cyanide | competitive inhibition with respect to nitrite | Spinacia oleracea | |
1.7.7.1 | p-chloromercuribenzoate | nitrite partially protects the inhibition | Spinacia oleracea |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.7.7.1 | Iron | contains about 2 mol of siroheme per mol of enzyme, 3 mol of iron per mol of enzyme (MW 61000), one in siroheme, one (Fe2-S2) | Spinacia oleracea |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.7.7.1 | 60000 | - |
gel filtration | Spinacia oleracea |
1.7.7.1 | 60000 | 63000 | - |
Spinacia oleracea |
1.7.7.1 | 62690 | - |
amino acid composition | Spinacia oleracea |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.7.1 | Spinacia oleracea | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.7.1 | homogeneity | Spinacia oleracea |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.7.7.1 | 107.8 | - |
- |
Spinacia oleracea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.7.1 | nitrite + reduced ferredoxin | - |
Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.7.7.1 | siroheme | prosthetic group | Spinacia oleracea | |
1.7.7.1 | siroheme | 1 mol siroheme per mol of enzyme | Spinacia oleracea |