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Literature summary extracted from

  • Vega, J.M.; Kamin, H.
    Spinach nitrite reductase. Purification and properties of a siroheme-containing iron-sulfur enzyme (1977), J. Biol. Chem., 252, 896-909.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.7.1 CO forms a complex with the reduced enzyme Spinacia oleracea
1.7.7.1 cyanide competitive inhibition with respect to nitrite Spinacia oleracea
1.7.7.1 p-chloromercuribenzoate nitrite partially protects the inhibition Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.7.1 Iron contains about 2 mol of siroheme per mol of enzyme, 3 mol of iron per mol of enzyme (MW 61000), one in siroheme, one (Fe2-S2) Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.7.1 60000
-
gel filtration Spinacia oleracea
1.7.7.1 60000 63000
-
Spinacia oleracea
1.7.7.1 62690
-
amino acid composition Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.1 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.1 homogeneity Spinacia oleracea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.7.1 107.8
-
-
Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.1 nitrite + reduced ferredoxin
-
Spinacia oleracea ammonia + oxidized ferredoxin
-
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Cofactor

EC Number Cofactor Comment Organism Structure
1.7.7.1 siroheme prosthetic group Spinacia oleracea
1.7.7.1 siroheme 1 mol siroheme per mol of enzyme Spinacia oleracea