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Literature summary extracted from

  • Arizmendi, J.M.; Serra, J.L.
    Purification and some properties of the nitrite reductase from the cyanobacterium Phormidium laminosum (1990), Biochim. Biophys. Acta, 1040, 237-244.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.7.1 bathophenanthroline slight Phormidium laminosum
1.7.7.1 cyanide 92% inhibition at 0.1 mM Phormidium laminosum
1.7.7.1 hydroxylamine 34% inhibition at 10 mM Phormidium laminosum
1.7.7.1 N-ethylmaleimide 47% inhibition at 1 mM Phormidium laminosum
1.7.7.1 NaN3 slight Phormidium laminosum
1.7.7.1 p-hydroxymercuribenzoate
-
 Phormidium laminosum
1.7.7.1 SO32- 24% inhibition at 10 mM Phormidium laminosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.7.1 0.022
-
 oxidized ferredoxin
-
 Phormidium laminosum
1.7.7.1 0.04
-
 nitrite
-
 Phormidium laminosum
1.7.7.1 0.215
-
 methyl viologen
-
 Phormidium laminosum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.7.1 chloroplast
-
 Phormidium laminosum 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.7.1 54000
-
 gel filtration Phormidium laminosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.7.1 nitrite + reduced ferredoxin Phormidium laminosum
-
 ammonia + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.1 Phormidium laminosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.1
-
 Phormidium laminosum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.7.1 625
-
-
 Phormidium laminosum

Storage Stability

EC Number Storage Stability Organism
1.7.7.1 -20°C, several months Phormidium laminosum
1.7.7.1 4°C, 1 week Phormidium laminosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.1 NH3 + H2O + oxidized ferredoxin
-
 Phormidium laminosum nitrite + reduced ferredoxin + H+
-
 r
1.7.7.1 nitrite + reduced ferredoxin
-
 Phormidium laminosum ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin NADH Phormidium laminosum ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin NADPH Phormidium laminosum ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin other electron donor: methyl viologen Phormidium laminosum ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin other electron donors: flavodoxin Phormidium laminosum ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin not: flavin Phormidium laminosum ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin other electron donor: benzyl viologen Phormidium laminosum ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced methyl viologen
-
 Phormidium laminosum NH3 + H2O + oxidized methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.7.1 monomer 1 * 54000 SDS-PAGE Phormidium laminosum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.7.1 50
-
 increase of activity up to 50°C Phormidium laminosum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.7.1 40
-
 thermostable up to 40°C Phormidium laminosum
1.7.7.1 60
-
 10 min, more than 95% loss of activity Phormidium laminosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.7.1 7.3
-
 phosphate buffer Phormidium laminosum
1.7.7.1 7.6
-
 Tris-HCl buffer Phormidium laminosum

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.7.1 siroheme prosthetic group Phormidium laminosum