Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Märki, F.; Martius, C.
    Vitamin K-Reduktase, Darstellung und eigenschaften (1960), Biochem. Z., 333, 111-135.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.2 2-(4'-chlorophenyl)-indan-1,3-dione 0.0002 mM, 50% inhibition Bos taurus
1.6.5.2 3,3'-methylene-bis(4-hydroxycoumarin)
-
 Bos taurus
1.6.5.2 3,3'-methylene-bis(4-hydroxycoumarin)
-
 Rattus norvegicus
1.6.5.2 3-(alpha-phenylpropyl)-4-hydroxycoumarin 0.01 mM, 50% inhibition Bos taurus
1.6.5.2 3-[alpha-(4'-nitrophenyl)-beta-acetylethyl]-4-hydroxycoumarin 0.02 mM, 50% inhibition Bos taurus
1.6.5.2 4,4'-dichlorodicoumarol 0.001 mM, 50% inhibition Bos taurus
1.6.5.2 4-Hydroxycoumarin
-
 Bos taurus
1.6.5.2 4-Methoxycoumarin
-
 Bos taurus
1.6.5.2 Dicoumarol dimethylether 0.0005, 50% inhibition Bos taurus
1.6.5.2 ethyl bis(2-hydroxy-4-oxo-4H-chromen-3-yl)acetate
-
 Bos taurus
1.6.5.2 methylene-5,5'-bis(4,6-dioxo-2-methyldihydropyran) 0.003 mM, 50% inhibition Bos taurus
1.6.5.2 additional information not inhibited by iodoacetate, 4-chloromercuribenzoate, Cu2+, FeSO4, FeCl3, CdSO4, ZnSO4, CuSO4, EDTA, alpha,alpha'-dipyridiyl, o-phenanthroline, dimethylglyoxime, dithizon, NaCN, amytal, veronal, antimycin, 2,4-dinitrophenol and thyroxin Bos taurus
1.6.5.2 additional information
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.2 52000
-
 sedimentation equilibrium, sedimentation velocity Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.5.2 vitamin K + NAD(P)H + H+ Bos taurus
-
 reduced vitamin K + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Bos taurus
-
-
-
1.6.5.2 Gallus gallus
-
-
-
1.6.5.2 Oryctolagus cuniculus
-
-
-
1.6.5.2 Rattus norvegicus
-
-
-
1.6.5.2 Sus scrofa
-
-
-
1.6.5.2 Tinca vulgaris
-
-
-
1.6.5.2 Xenopus laevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.2 heart
-
 Rattus norvegicus
-
1.6.5.2 heart
-
 Bos taurus
-
1.6.5.2 heart
-
 Oryctolagus cuniculus
-
1.6.5.2 kidney
-
 Gallus gallus
-
1.6.5.2 kidney
-
 Rattus norvegicus
-
1.6.5.2 kidney
-
 Sus scrofa
-
1.6.5.2 kidney
-
 Bos taurus
-
1.6.5.2 kidney
-
 Oryctolagus cuniculus
-
1.6.5.2 liver
-
 Gallus gallus
-
1.6.5.2 liver
-
 Rattus norvegicus
-
1.6.5.2 liver
-
 Sus scrofa
-
1.6.5.2 liver
-
 Bos taurus
-
1.6.5.2 liver
-
 Oryctolagus cuniculus
-
1.6.5.2 liver
-
 Xenopus laevis
-
1.6.5.2 liver
-
 Tinca vulgaris
-
1.6.5.2 lung
-
 Rattus norvegicus
-
1.6.5.2 lung
-
 Bos taurus
-
1.6.5.2 lung
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.2 additional information
-
 1400000.0, 1 unit is defined as the amount of enzyme causing a decrease in absorbance at 340 nm of 0.001/min Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.6.5.2 -20°C, some weeks Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 1,4-dimethoxymethylbenzoquinone + NAD(P)H
-
 Bos taurus 1,4-dimethoxymethylhydrobenzoquinone + NAD(P)+
-
 ?
1.6.5.2 1,4-naphthoquinone + NAD(P)H
-
 Bos taurus 1,4-naphthohydroquinone + NAD(P)+
-
 ?
1.6.5.2 1,4-tetramethylbenzoquinone + NAD(P)H
-
 Bos taurus 1,4-tetramethylhydrobenzoquinone + NAD(P)+
-
 ?
1.6.5.2 2,3-dimethyl-1,4-naphthoquinone + NAD(P)H
-
 Bos taurus 2,3-dimethyl-1,4-hydronaphthoquinone + NAD(P)+
-
 ?
1.6.5.2 2-ethyl-1,4-naphthoquinone + NAD(P)H
-
 Bos taurus 2-ethyl-1,4-hydronaphthoquinone + NAD(P)+
-
 ?
1.6.5.2 2-methyl-1,4-naphthoquinone + NAD(P)H artificial acceptor, trivial name menadione Rattus norvegicus 2-methyl-1,4-naphthoquinol + NAD(P)+
-
 ?
1.6.5.2 2-methyl-1,4-naphthoquinone + NAD(P)H artificial acceptor, trivial name menadione Bos taurus 2-methyl-1,4-naphthoquinol + NAD(P)+
-
 ?
1.6.5.2 2-methyl-1,4-naphthoquinone + NAD(P)H
-
 Bos taurus 2-methyl-1,4-dihydronaphthoquinone + NAD(P)+
-
 ?
1.6.5.2 benzoquinone + 2 NAD(P)H + H+
-
 Bos taurus benzohydroquinone + 2 NAD(P)+
-
 ?
1.6.5.2 K3[Fe(CN)6] + NAD(P)H no activity with lipoic acid, cytochrome c and vitamin K1 Bos taurus K4[Fe(CN)6] + NAD(P)+
-
 ?
1.6.5.2 NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Rattus norvegicus NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.6.5.2 NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Bos taurus NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.6.5.2 vitamin K + NAD(P)H + H+
-
 Bos taurus reduced vitamin K + NAD(P)+
-
 ?
1.6.5.2 vitamin K2 + NAD(P)H
-
 Bos taurus reduced vitamin K2 + NAD(P)+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.5.2 11700
-
 menadione
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.5.2 5.7
-
 menadione Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.5.2 4.8 9.8
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 FAD enzyme contains FAD Rattus norvegicus
1.6.5.2 FAD enzyme contains FAD Bos taurus
1.6.5.2 FAD contains 1 mol of FAD per mol of enzyme Rattus norvegicus
1.6.5.2 FAD contains 1 mol of FAD per mol of enzyme Bos taurus
1.6.5.2 NADH
-
 Rattus norvegicus
1.6.5.2 NADH
-
 Bos taurus
1.6.5.2 NADPH
-
 Rattus norvegicus
1.6.5.2 NADPH
-
 Bos taurus