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Literature summary extracted from

  • Wehrfritz, J.M.; Reilly, A.; Spiro, S.; Richardson, D.J.
    Purification of hydroxylamine oxidase from Thiosphaera pantotropha. Identification of electron acceptors that couple heterotrophic nitrification to aerobic denitrification (1993), FEBS Lett., 335, 246-250.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.3.6 0.01
-
 ferricytochrome c551 pH 7.8, 25°C Paracoccus denitrificans
1.7.3.6 0.033
-
 pseudoazurin pH 7.8, 25°C Paracoccus denitrificans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.3.6 periplasm
-
 Paracoccus denitrificans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.3.6 Fe non-heme iron enzyme, the enzyme could possess a non-heme ferric iron centre that is lost when the 20000 Da polypeptide is subjected to SDS-PAGE, but that can be reconstituted by addition of Fe3+ ions to the refolded enzyme Paracoccus denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.3.6 20000
-
 x * 20000, SDS-PAGE Paracoccus denitrificans

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.6 Paracoccus denitrificans
-
 i.e. Thiosphaera pantotropha, heterotrophic nitrifying bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.3.6 partial Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.6 hydroxylamine + ferricytochrome c551
-
 Paracoccus denitrificans nitroxyl + ferrocytochrome c551 + H+
-
 ?
1.7.3.6 hydroxylamine + pseudoazurin
-
 Paracoccus denitrificans nitrite + reduced pseudoazurin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.3.6 ? x * 20000, SDS-PAGE Paracoccus denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.3.6 additional information non-heme iron enzyme Paracoccus denitrificans