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Literature summary extracted from

  • Smith, E.J.; Van Loon, E.J.
    1,2-Dimethyl-4-(p-carboxyphenylazo)-5-hydroxybenzene. A convenient substrate for the measurement of azo reductase (1969), Anal. Biochem., 31, 315-320.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.1.6 furazolidone
-
 Rattus norvegicus
1.7.1.6 Nitrofurantoin
-
 Rattus norvegicus
1.7.1.6 nitrofurazone
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.6 0.12
-
 1,2-dimethyl-4-(p-carboxyphenylazo)-5-hydroxy-benzene
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Canis lupus familiaris
-
-
-
1.7.1.6 Cavia porcellus
-
-
-
1.7.1.6 Gallus gallus
-
-
-
1.7.1.6 Microtus pennsylvanicus
-
 meadow vole
-
1.7.1.6 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.6 liver
-
 Gallus gallus
-
1.7.1.6 liver
-
 Cavia porcellus
-
1.7.1.6 liver
-
 Rattus norvegicus
-
1.7.1.6 liver
-
 Canis lupus familiaris
-
1.7.1.6 liver
-
 Microtus pennsylvanicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.6 0.045
-
 activity in liver supernatant fraction Canis lupus familiaris
1.7.1.6 0.053
-
 activity in liver supernatant fraction Gallus gallus
1.7.1.6 0.06
-
 activity in liver supernatant fraction Microtus pennsylvanicus
1.7.1.6 0.067
-
 activity in liver supernatant fraction Rattus norvegicus
1.7.1.6 0.087
-
 activity in liver supernatant fraction Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 1,2-dimethyl-4(p-carboxyphenylazo)-5-hydroxybenzene + NADPH
-
 Gallus gallus 1,2-dimethyl-4-amino-5-hydroxybenzene + p-carboxyphenylamine + NADP+
-
 ?
1.7.1.6 1,2-dimethyl-4(p-carboxyphenylazo)-5-hydroxybenzene + NADPH
-
 Cavia porcellus 1,2-dimethyl-4-amino-5-hydroxybenzene + p-carboxyphenylamine + NADP+
-
 ?
1.7.1.6 1,2-dimethyl-4(p-carboxyphenylazo)-5-hydroxybenzene + NADPH
-
 Rattus norvegicus 1,2-dimethyl-4-amino-5-hydroxybenzene + p-carboxyphenylamine + NADP+
-
 ?
1.7.1.6 1,2-dimethyl-4(p-carboxyphenylazo)-5-hydroxybenzene + NADPH
-
 Canis lupus familiaris 1,2-dimethyl-4-amino-5-hydroxybenzene + p-carboxyphenylamine + NADP+
-
 ?
1.7.1.6 1,2-dimethyl-4(p-carboxyphenylazo)-5-hydroxybenzene + NADPH
-
 Microtus pennsylvanicus 1,2-dimethyl-4-amino-5-hydroxybenzene + p-carboxyphenylamine + NADP+
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH
-
 Gallus gallus N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH
-
 Cavia porcellus N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH
-
 Rattus norvegicus N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH
-
 Canis lupus familiaris N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH
-
 Microtus pennsylvanicus N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 FAD increases activity Rattus norvegicus