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Literature summary extracted from

  • Zimmermann, T.; Kulla, H.G.; Leisinger, T.
    Properties of purified Orange II azoreductase, the enzyme initiating azo dye degradation by Pseudomonas KF46 (1982), Eur. J. Biochem., 129, 197-203.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.6 Orange II substrate inhibition at high concentration Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.6 0.0009
-
 1-phenylazo-2-hydroxy-6-sulfonaphthalene
-
 Pseudomonas sp.
1.7.1.6 0.001
-
 1-(4'-sulfophenylazo)-2-naphthol
-
 Pseudomonas sp.
1.7.1.6 0.0013
-
 1-(2'-methyl-4'-sulfophenylazo)-2-naphthol
-
 Pseudomonas sp.
1.7.1.6 0.0014
-
 1-phenylazo-2-hydroxy-6-carboxynaphthalene
-
 Pseudomonas sp.
1.7.1.6 0.0015
-
 1-(3'-carboxyphenylazo)-2-naphthol
-
 Pseudomonas sp.
1.7.1.6 0.0018
-
 1-(3'-sulfophenylazo)-2-naphthol
-
 Pseudomonas sp.
1.7.1.6 0.0018
-
 1-(4'-nitrophenylazo)-2-hydroxy-6-sulfonaphthalene
-
 Pseudomonas sp.
1.7.1.6 0.005
-
 NADPH
-
 Pseudomonas sp.
1.7.1.6 0.0052
-
 1-(4'-phosphophenylazo)-2-naphthol
-
 Pseudomonas sp.
1.7.1.6 0.008
-
 1-(4'-carboxyphenylazo)-2-naphthol
-
 Pseudomonas sp.
1.7.1.6 0.0105
-
 1-(3'-phosphophenylazo)-2-naphthol
-
 Pseudomonas sp.
1.7.1.6 0.013
-
 1-(4'-carboxyphenylazo)-2-hydroxy-6-carboxynaphthalene
-
 Pseudomonas sp.
1.7.1.6 0.0132
-
 1-(4'-sulfophenylazo)-2-hydroxy-6-carboxynaphthalene
-
 Pseudomonas sp.
1.7.1.6 0.0137
-
 1-(2'-sulfophenylazo)-2-naphthol
-
 Pseudomonas sp.
1.7.1.6 0.0148
-
 1-(4'-sulfoaminophenylazo)-2-naphthol
-
 Pseudomonas sp.
1.7.1.6 0.017
-
 1-(4'-sulfophenylazo)-2-hydroxy-6-sulfonaphthalene
-
 Pseudomonas sp.
1.7.1.6 0.0198
-
 1-(4'-carboxyphenylazo)-2-hydroxy-6-sulfonaphthalene
-
 Pseudomonas sp.
1.7.1.6 0.18
-
 NADH
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.6 30000
-
 1 * 30000, strain KF46, SDS-PAGE, Orange II azoreductase Pseudomonas sp.
1.7.1.6 32000
-
 strain KF 46, gel filtration, Orange II azoreductase Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Pseudomonas sp.
-
 Orange II azoreductase induced by both Orange II and carboxy-Orange II
-
1.7.1.6 Pseudomonas sp. KF46
-
 Orange II azoreductase induced by both Orange II and carboxy-Orange II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.6
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.6 2868
-
 orange I azoreductase, strain KF24 Pseudomonas sp.
1.7.1.6 17830
-
 orange II azoreductase, strain KF46 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.7.1.6 -20°C, 50% glycerol, several months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 1-(2'-methyl-4'-sulfophenylazo)-2-naphthol + NADH + H+
-
 Pseudomonas sp. 4-amino-2-methyl-1-benzenesulfonic acid + NAD+ + 1-amino-2-hydroxynaphthalene
-
 r
1.7.1.6 1-(2'-methyl-4'-sulfophenylazo)2-naphthol + NAD(P)H
-
 Pseudomonas sp. 4-amino-2-methyl-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 1-(2'-sulfophenylazo)-2-naphthol + NADH + H+
-
 Pseudomonas sp. ?
-
 r
1.7.1.6 1-(3'-carboxyphenylazo)-2-naphthol + NAD(P)H
-
 Pseudomonas sp. 3-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 1-(3'-phosphophenylazo)-2-naphthol + NAD(P)H
-
 Pseudomonas sp. 3-amino-1-benzenephosphate + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 1-(3'-sulfophenylazo)-2-naphthol + NAD(P)H
-
 Pseudomonas sp. 3-amino-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 1-(4'-carboxyphenylazo)-2-hydroxy-6-carboxynaphthalene + NADH + H+
-
 Pseudomonas sp. ?
-
 r
1.7.1.6 1-(4'-carboxyphenylazo)-2-hydroxy-6-sulfonaphthalene + NAD(P)H
-
 Pseudomonas sp. 4-carboxyphenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene
-
 ?
1.7.1.6 1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H
-
 Pseudomonas sp. 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H
-
 Pseudomonas sp. KF46 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 1-(4'-carboxyphenylazo)-2-naphthol + NADH + H+ i.e. carboxy-Orange II Pseudomonas sp. 4-aminobenzoate + NAD+ + ?
-
 r
1.7.1.6 1-(4'-nitrophenylazo)-2-hydroxy-6-sulfonaphthalene + NAD(P)H
-
 Pseudomonas sp. 4-nitrophenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene
-
 ?
1.7.1.6 1-(4'-phosphophenylazo)-2-naphthol + NAD(P)H
-
 Pseudomonas sp. 4-amino-1-benzenephosphate + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 1-(4'-phosphophenylazo)-2-naphthol + NAD(P)H
-
 Pseudomonas sp. KF46 4-amino-1-benzenephosphate + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 1-(4'-sulfonaminophenylazo-)2-naphthol + NAD(P)H
-
 Pseudomonas sp. 4-amino-1-benzenesulfonamine + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 1-(4'-sulfonaminophenylazo-)2-naphthol + NAD(P)H
-
 Pseudomonas sp. KF46 4-amino-1-benzenesulfonamine + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 1-(4'-sulfophenylazo)-2-hydroxy-6-carboxynaphthalene + NAD(P)H
-
 Pseudomonas sp. 4-sulfophenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-carboxynaphthalene
-
 ?
1.7.1.6 1-(4'-sulfophenylazo)-2-hydroxy-6-sulfonaphthalene + NAD(P)H
-
 Pseudomonas sp. 4-sulfophenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene
-
 ?
1.7.1.6 1-phenylazo-2-hydroxy-6-carboxynaphthalene + NAD(P)H
-
 Pseudomonas sp. phenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-carboxynaphthalene
-
 ?
1.7.1.6 1-phenylazo-2-hydroxy-6-sulfonaphthalene + NAD(P)H
-
 Pseudomonas sp. phenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH Orange II azoreductase: requirement for substrates with a 2-naphthol moiety Pseudomonas sp. N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH Orange II azoreductase: requirement for substrates with a 2-naphthol moiety Pseudomonas sp. KF46 N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
1.7.1.6 4-[(2-hydroxy-1-naphthalenyl)azo]benzene sulfonic acid + NAD(P)H trivial name Orange II Pseudomonas sp. 4-amino-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 4-[(2-hydroxy-1-naphthalenyl)azo]benzene sulfonic acid + NAD(P)H trivial name Orange II Pseudomonas sp. KF46 4-amino-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 Solar Orange + NADPH
-
 Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.6 monomer 1 * 30000, strain KF46, SDS-PAGE, Orange II azoreductase Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.6 45
-
-
 Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.7.1.6 20 50 20 C: about 25% activity with carboxy-Orange II, about 30% activity with Orange II, 50 C: about 95% activity with carboxy-Orange II, about 80% activity with Orange II Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.6 35
-
 stable below Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.6 5 8 Orange II + NADH Pseudomonas sp.
1.7.1.6 6.5
-
 carboxy-Orange II + NADH Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 NADH
-
 Pseudomonas sp.
1.7.1.6 NADPH
-
 Pseudomonas sp.