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Literature summary extracted from

  • Cresswell, C.F.; Hageman, R.H.; Hewitt, E.J.; Hucklesby, D.P.
    The reduction of nitrate, nitrite and hydroxylamine to ammonia by enzymes from Curcubita pepo L. in the presence of reduced benzyl viologen as electron donor (1965), Biochem. J., 94, 40-53.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.99.1 2,2'-bipyridyl 10% stimulation at 1 mM Cucurbita pepo
1.7.99.1 8-hydroxyquinoline 5% stimulation at 1 mM Cucurbita pepo

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.99.1 cyanide 100% inhibition at 5 mM Cucurbita pepo
1.7.99.1 hydrazine hydrazine sulfate inhibits 10-15% at 4 mM Cucurbita pepo
1.7.99.1 nitrite
-
Cucurbita pepo
1.7.99.1 p-chloromercuribenzoate 47% inhibition at 0.5 mM Cucurbita pepo
1.7.99.1 phenylmercuric acetate 73% inhibition at 0.5 mM Cucurbita pepo
1.7.99.1 pyruvate 20% inhibition at 3.5 mM Cucurbita pepo

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.99.1 0.0075
-
reduced benzyl viologen
-
Cucurbita pepo
1.7.99.1 0.05 0.9 hydroxylamine
-
Cucurbita pepo

Organism

EC Number Organism UniProt Comment Textmining
1.7.99.1 Cucurbita pepo
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.99.1 hydroxylamine + reduced acceptor reduced benzyl viologen as electron donor Cucurbita pepo ammonia + oxidized acceptor
-
?
1.7.99.1 nitrate + reduced benzyl viologen
-
Cucurbita pepo nitrite + H2O + oxidized benzyl viologen
-
?