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Literature summary extracted from
- Hydroxylamine reductase from Pseudomonas aeruginosa (1961), Biochim. Biophys. Acta, 49, 361-368.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.99.1 | Cu2+ | inhibition of dialyzed enzyme at 0.0005 mM | Pseudomonas aeruginosa |  |
| 1.7.99.1 | hydrazine | competitive inhibition | Pseudomonas aeruginosa | |
| 1.7.99.1 | Zn2+ | inhibition of dialyzed enzyme at 0.0005 mM | Pseudomonas aeruginosa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.7.99.1 | 0.0000004 | - |
hydroxylamine | - |
Pseudomonas aeruginosa | |
| 1.7.99.1 | 0.00001 | - |
FADH2 | - |
Pseudomonas aeruginosa | |
| 1.7.99.1 | 0.000012 | - |
reduced pyocyanine | - |
Pseudomonas aeruginosa | |
| 1.7.99.1 | 0.000044 | - |
reduced methylene blue | - |
Pseudomonas aeruginosa | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.99.1 | Co2+ | can substitute for Mn2+, only 10% as effective | Pseudomonas aeruginosa | |
| 1.7.99.1 | Mn2+ | required for maximal activity | Pseudomonas aeruginosa | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.7.99.1 | Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.7.99.1 | - |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.7.99.1 | 2.87 | - |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.7.99.1 | hydroxylamine + reduced acceptor | with pyocyanine or reduced methylene blue as electron donor | Pseudomonas aeruginosa | ammonia + oxidized acceptor | - |
? | |
| 1.7.99.1 | nitrate + FADH2 | - |
Pseudomonas aeruginosa | nitrite + FAD + H2O | - |
r | |
| 1.7.99.1 | nitrate + reduced methylene blue | - |
Pseudomonas aeruginosa | nitrite + H2O + oxidized methylene blue | - |
r | |
| 1.7.99.1 | nitrate + reduced pyocyanine | - |
Pseudomonas aeruginosa | nitrite + H2O + oxidized pyocyanine | - |
r | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.7.99.1 | 37 | - |
- |
Pseudomonas aeruginosa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.7.99.1 | 7.8 | - |
- |
Pseudomonas aeruginosa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.99.1 | FAD | flavoprotein, contains 0.9 nmol FAD per mg protein | Pseudomonas aeruginosa | |
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Release 2023.1 (January 2023)
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