Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kimura, S.; Emi, Y.; Ikushiro, S.; Iyanagi, T.
    Systematic mutations of highly conserved His49 and carboxyl-terminal of recombinant porcine liver NADH-cytochrome b5 reductase solubilized domain (1999), Biochim. Biophys. Acta, 1430, 290-301.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.2 expression of wild-type, H49A, H49E, H49K, H49Y, and DELTAF272 mutant enzymes in Escherichia coli Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
1.6.2.2 H49A reduced kcat Sus scrofa
1.6.2.2 H49E elevated Km value for cytochrome b5, strongly reduced kcat Sus scrofa
1.6.2.2 H49K reduced Km value for cytochrome b5 Sus scrofa
1.6.2.2 H49Y similar to wild-type Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.0003
-
 NADH H49E mutant enzyme Sus scrofa
1.6.2.2 0.0006
-
 ferricyanide H49K mutant enzyme Sus scrofa
1.6.2.2 0.00064
-
 NADH G273 mutant enzyme Sus scrofa
1.6.2.2 0.00084
-
 NADH DELTAF272 mutant enzyme Sus scrofa
1.6.2.2 0.0015
-
 NADH recombinant wild-type enzyme Sus scrofa
1.6.2.2 0.0016
-
 NADH native enzyme Sus scrofa
1.6.2.2 0.0019
-
 NADH H49A mutant enzyme Sus scrofa
1.6.2.2 0.0022
-
 ferricyanide H49E mutant enzyme Sus scrofa
1.6.2.2 0.0025
-
 ferricyanide native enzyme Sus scrofa
1.6.2.2 0.0025
-
 cytochrome b5 G273 mutant enzyme Sus scrofa
1.6.2.2 0.0026
-
 ferricyanide H49Y mutant enzyme Sus scrofa
1.6.2.2 0.0027
-
 NADH H49Y mutant enzyme Sus scrofa
1.6.2.2 0.0028
-
 ferricyanide recombinant wild-type enzyme Sus scrofa
1.6.2.2 0.0028
-
 ferricyanide H49A mutant enzyme Sus scrofa
1.6.2.2 0.0031
-
 cytochrome b5 H49K mutant enzyme Sus scrofa
1.6.2.2 0.0052
-
 ferricyanide DELTAF272 mutant enzyme Sus scrofa
1.6.2.2 0.0052
-
 ferricyanide G273 mutant enzyme Sus scrofa
1.6.2.2 0.0088
-
 cytochrome b5 native enzyme Sus scrofa
1.6.2.2 0.0089
-
 cytochrome b5 H49A mutant enzyme Sus scrofa
1.6.2.2 0.0091
-
 cytochrome b5 recombinant wild-type enzyme Sus scrofa
1.6.2.2 0.01
-
 NADH H49K mutant enzyme Sus scrofa
1.6.2.2 0.011
-
 cytochrome b5 H49Y mutant enzyme Sus scrofa
1.6.2.2 0.017
-
 cytochrome b5 DELTAF272 mutant enzyme Sus scrofa
1.6.2.2 0.03
-
 cytochrome b5 H49E mutant enzyme Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2 recombinant wild-type, H49A, H49E, H49K, H49Y, and DELTAF272 mutant enzyme Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 NADH + ferricytochrome b5
-
 Sus scrofa NAD+ + H+ + ferrocytochrome b5
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.2 53
-
 NADH G273 mutant enzyme Sus scrofa
1.6.2.2 57
-
 ferricytochrome b5 G273 mutant enzyme Sus scrofa
1.6.2.2 76
-
 ferricyanide G273 mutant enzyme Sus scrofa
1.6.2.2 77
-
 NADH H49E mutant enzyme Sus scrofa
1.6.2.2 110
-
 ferricyanide H49E mutant enzyme Sus scrofa
1.6.2.2 160
-
 ferricytochrome b5 H49E mutant enzyme Sus scrofa
1.6.2.2 310
-
 NADH DELTAF272 mutant enzyme Sus scrofa
1.6.2.2 380
-
 ferricytochrome b5 H49A mutant enzyme Sus scrofa
1.6.2.2 390
-
 NADH native enzyme Sus scrofa
1.6.2.2 400
-
 ferricyanide DELTAF272 mutant enzyme Sus scrofa
1.6.2.2 420
-
 ferricytochrome b5 H49K mutant enzyme Sus scrofa
1.6.2.2 440
-
 NADH H49A mutant enzyme Sus scrofa
1.6.2.2 450
-
 ferricytochrome b5 DELTAF272 mutant enzyme Sus scrofa
1.6.2.2 470
-
 ferricyanide H49A mutant enzyme Sus scrofa
1.6.2.2 490
-
 ferricyanide H49K mutant enzyme Sus scrofa
1.6.2.2 510
-
 ferricytochrome b5 native enzyme Sus scrofa
1.6.2.2 560
-
 ferricytochrome b5 recombinant wild-type enzyme Sus scrofa
1.6.2.2 570
-
 NADH recombinant wild-type enzyme Sus scrofa
1.6.2.2 580
-
 ferricytochrome b5 H49Y mutant enzyme Sus scrofa
1.6.2.2 630
-
 ferricyanide native enzyme Sus scrofa
1.6.2.2 710
-
 NADH H49K mutant enzyme Sus scrofa
1.6.2.2 727
-
 ferricyanide H49Y mutant enzyme Sus scrofa
1.6.2.2 750
-
 NADH H49Y mutant enzyme Sus scrofa
1.6.2.2 810
-
 ferricyanide recombinant wild-type enzyme Sus scrofa