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Literature summary extracted from

  • Lee, J.Y.; Kim, Y.H.; Lee, S.J.
    Purification and comparison of NADH-cytochrome b5 reductase from mitochondrial outer membrane of bovine heart and turnip (1998), Bull. Chem. Soc. Kor., 19, 160-164.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 2.17
-
 NADH
-
 Bos taurus
1.6.2.2 2.34
-
 NADPH
-
 Bos taurus
1.6.2.2 3.32
-
 NADH
-
 Brassica napus
1.6.2.2 3.85
-
 ferricyanide
-
 Bos taurus
1.6.2.2 4.35
-
 ferricyanide
-
 Brassica napus
1.6.2.2 7.69
-
 NADPH
-
 Brassica napus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.2 membrane
-
 Bos taurus 16020
-
1.6.2.2 membrane
-
 Brassica napus 16020
-
1.6.2.2 mitochondrion
-
 Brassica napus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.2 35000
-
 x * 35000, SDS-PAGE Bos taurus
1.6.2.2 35000
-
 x * 35000, SDS-PAGE Brassica napus

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Bos taurus
-
-
-
1.6.2.2 Brassica napus
-
 turnip
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2 DEAE-cellulose, DEAE-Sephadex, hydroxyapatite Brassica napus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.2 additional information
-
 28.6 units, 1 unit is defined as the amount of enzyme changing 0.001 of DELTAAbsorbance/min Bos taurus
1.6.2.2 additional information
-
 41.25 units, 1 unit is defined as the amount of enzyme changing 0.001 of DELTAAbsorbance/min Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricytochrome b5 + NADH
-
 Bos taurus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH artificial acceptor: ferricyanide, high reactivity with NADPH as electron donor Brassica napus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 NADH + ferricyanide
-
 Brassica napus NAD+ + H+ + ferrocyanide
-
 r
1.6.2.2 NADPH + ferricyanide
-
 Bos taurus NADP+ + H+ + ferrocyanide
-
 r
1.6.2.2 NADPH + ferricyanide
-
 Brassica napus NADP+ + H+ + ferrocyanide
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.2.2 ? x * 35000, SDS-PAGE Bos taurus
1.6.2.2 ? x * 35000, SDS-PAGE Brassica napus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 NADPH
-
 Brassica napus