Literature summary extracted from
Barham, H.M.; Inglis, R.; Chinje, E.C.; Stratford, I.J.
Development and validation of a spectrophotometric assay for measuring the activity of NADH: cytochrome b5 reductase in human tumour cells (1996), Br. J. Cancer, 74, 1188-1193.
Application
EC Number |
Application |
Comment |
Organism |
---|
1.6.2.2 |
medicine |
potentially an important enzyme required for the reductive activation of bioreductive drugs that can be used in the treatment of solid tumours |
Homo sapiens |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.6.2.2 |
Homo sapiens |
- |
different cancer cell lines |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.6.2.2 |
0.036 |
0.109 |
activity in lysates of different cancer cell lines |
Homo sapiens |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.6.2.2 |
2 ferricytochrome b5 + NADH |
artificial electron acceptor in the presence of menadione: cytochrome c |
Homo sapiens |
2 ferrocytochrome b5 + NAD+ + H+ |
- |
? |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.6.2.2 |
6.8 |
- |
reduction of cytochrome c |
Homo sapiens |