EC Number | Cloned (Comment) | Organism |
---|---|---|
1.6.2.2 | expression of wild-type, K110A, K110M and K110R mutant enzymes in Escherichia coli | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.6.2.2 | K110A | 200fold elevated Km value for NADH, 85% of kcat | Homo sapiens |
1.6.2.2 | K110M | 1120fold elevated Km value for NADH | Homo sapiens |
1.6.2.2 | K110R | similar kinetic properties as wild-type | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.2.2 | 0.0006 | - |
NADH | recombinant wild-type enzyme | Homo sapiens | |
1.6.2.2 | 0.0023 | - |
NADH | recombinant K110R mutant enzyme | Homo sapiens | |
1.6.2.2 | 0.0066 | - |
ferricytochrome b5 | recombinant wild-type enzyme | Homo sapiens | |
1.6.2.2 | 0.0142 | - |
ferricytochrome b5 | recombinant K110A mutant enzyme | Homo sapiens | |
1.6.2.2 | 0.0167 | - |
ferricytochrome b5 | recombinant K110R mutant enzyme | Homo sapiens | |
1.6.2.2 | 0.0285 | - |
ferricytochrome b5 | recombinant K110M mutant enzyme | Homo sapiens | |
1.6.2.2 | 0.121 | - |
NADH | recombinant K110A mutant enzyme | Homo sapiens | |
1.6.2.2 | 0.672 | - |
NADH | recombinant K110M mutant enzyme | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.2.2 | 2 ferricytochrome b5 + NADH | Homo sapiens | enzyme participates in methemoglobin reduction in erythrocytes, in other tissues it plays a role in elongation and desaturation of fatty acids, P-450 mediated drug metabolism and cholesterol biosynthesis as part of the microsomal electron transfer system | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.2.2 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.6.2.2 | recombinant wild-type, K110A, K110M and K110R mutant enzyme | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.2.2 | 2 ferricytochrome b5 + NADH | enzyme participates in methemoglobin reduction in erythrocytes, in other tissues it plays a role in elongation and desaturation of fatty acids, P-450 mediated drug metabolism and cholesterol biosynthesis as part of the microsomal electron transfer system | Homo sapiens | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
1.6.2.2 | NADH + ferricytochrome b5 | - |
Homo sapiens | NAD+ + H+ + ferrocytochrome b5 | - |
r |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.2.2 | 563 | - |
ferricytochrome b5 | recombinant K110M mutant enzyme | Homo sapiens | |
1.6.2.2 | 742 | - |
ferricytochrome b5 | recombinant K110A mutant enzyme | Homo sapiens | |
1.6.2.2 | 872 | - |
ferricytochrome b5 | recombinant wild-type enzyme | Homo sapiens | |
1.6.2.2 | 877 | - |
ferricytochrome b5 | recombinant K110R mutant enzyme | Homo sapiens |