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Literature summary extracted from

  • Kubota, S.; Yoshida, Y.; Kumaoka, H.
    Studies on the microsomal electron-transport system of anaerobically grown yeast. IV. Purification and characterization of NADH-cytochrome b5 reductase (1977), J. Biochem., 81, 187-195.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.6.2.2 unstable, 40% loss of activity at 0°C overnight, 1 mg/ml gelatin stabilizes, FAD protects against inactivation Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.2 1,10-phenanthroline
-
 Saccharomyces cerevisiae
1.6.2.2 2,2'-dipyridyl
-
 Saccharomyces cerevisiae
1.6.2.2 Hg2+
-
 Saccharomyces cerevisiae
1.6.2.2 K+ high ionic strength
-
 Saccharomyces cerevisiae
1.6.2.2 NAD+ competitive Saccharomyces cerevisiae
1.6.2.2 p-chloromercuribenzoate
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.0008
-
 ferricytochrome b5
-
 Saccharomyces cerevisiae
1.6.2.2 0.0008
-
 ferricytochrome b5 yeast cytochrome b5 Saccharomyces cerevisiae
1.6.2.2 0.0015
-
 ferricytochrome b5 calf cytochrome b5 Saccharomyces cerevisiae
1.6.2.2 0.0063
-
 NADH
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.2 endoplasmic reticulum
-
 Saccharomyces cerevisiae 5783
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.2 27000
-
 gel filtration Saccharomyces cerevisiae
1.6.2.2 28000
-
 1 * 28000, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Saccharomyces cerevisiae
-
 grown anaerobically
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.2 69.8
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricytochrome b5 + NADH specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol Saccharomyces cerevisiae 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 NADH + ferricytochrome b5
-
 Saccharomyces cerevisiae NAD+ + H+ + ferrocytochrome b5
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.2.2 monomer 1 * 28000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.2 5.6
-
 steady decrease above Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 NADH
-
 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.2.2 0.23
-
 NAD+
-
 Saccharomyces cerevisiae