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Literature summary extracted from

  • Badwey, J.A.; Tauber, A.I.; Karnovsky, M.L.
    Properties of NADH-cytochrome-b5 reductase from human neutrophils (1983), Blood, 62, 152-157.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.2.2 Detergents e.g. Triton X-100, activation Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.2 ADP 5 mM, 66% inhibition Homo sapiens
1.6.2.2 dicoumarol 0.3 mM, 57% inhibition Homo sapiens
1.6.2.2 N-ethylmaleimide 8 mM, 70% inhibition Homo sapiens
1.6.2.2 p-chloromercuribenzoate 0.005 mM, complete inhibition Homo sapiens
1.6.2.2 taurodeoxycholate 20 mM, 84% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.0384
-
 ferricyanide
-
 Homo sapiens
1.6.2.2 0.0478
-
 NADH
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.2 neutrophil
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.2 additional information
-
 0.00017 mmol ferricyanide reduced/min/10000000 cells, activity in neutrophils Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricyanide + NADH
-
 Homo sapiens 2 ferrocyanide + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol Homo sapiens 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH additional electron donors: deamino-NADH, 3-acetylpyridine-NADH Homo sapiens 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH artificial acceptors: p-benzoquinone, 5-hydroxy-1,4-naphthoquinone, nitroblue-tetrazolium Homo sapiens 2 ferrocytochrome b5 + NAD+ + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.2 5.7 8 rapid decrease above Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 NADH
-
 Homo sapiens