EC Number | General Stability | Organism |
---|---|---|
1.6.2.2 | inactivation during dialysis is prevented by NADH | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.6.2.2 | Acrynol | 0.1 mM, 75% inhibition | Bos taurus | |
1.6.2.2 | adenine nucleotides | - |
Bos taurus | |
1.6.2.2 | Atebrin | 1 mM, complete inhibition | Bos taurus | |
1.6.2.2 | Cl- | - |
Bos taurus | |
1.6.2.2 | iodoacetic acid | 5 mM, complete inhibition | Bos taurus | |
1.6.2.2 | N-ethylmaleimide | 10 mM, 89% inhibition | Bos taurus | |
1.6.2.2 | p-chloromercuribenzoate | 0.001 mM, complete inhibition | Bos taurus | |
1.6.2.2 | phosphate | competitive inhibition | Bos taurus | |
1.6.2.2 | Proflavin | 0.1 mM, 86% inhibition | Bos taurus | |
1.6.2.2 | taurodeoxycholate | - |
Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.2.2 | 0.0011 | - |
NADH | - |
Bos taurus | |
1.6.2.2 | 0.0042 | - |
ferricyanide | - |
Bos taurus | |
1.6.2.2 | 0.0143 | - |
ferricytochrome b5 | - |
Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.6.2.2 | endoplasmic reticulum | - |
Bos taurus | 5783 | - |
1.6.2.2 | membrane | membrane-binding domain resides in the NH2-terminal region | Bos taurus | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.6.2.2 | 31000 | - |
x * 31000, SDS-PAGE | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.2.2 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.6.2.2 | lysosome-solubilization | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.6.2.2 | brain | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.6.2.2 | 137 | - |
- |
Bos taurus |
EC Number | Storage Stability | Organism |
---|---|---|
1.6.2.2 | 0°C, at least 1 week | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.2.2 | 2 ferricytochrome b5 + NADH | specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol | Bos taurus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
1.6.2.2 | 2 ferricytochrome b5 + NADH | no acceptors: ubiquinone-30, menadione, dihydrofolate, lipoamide | Bos taurus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
1.6.2.2 | 2 ferricytochrome b5 + NADH | poor donor: NADPH | Bos taurus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
1.6.2.2 | NADH + ferricytochrome b5 | - |
Bos taurus | NAD+ + H+ + ferrocytochrome b5 | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.6.2.2 | ? | x * 31000, SDS-PAGE | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.6.2.2 | 7 | 7.5 | - |
Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.6.2.2 | NADH | - |
Bos taurus |