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Literature summary extracted from

  • Tamura, M.; Yubisui, T.; Takeshita, M.
    Microsomal NADH-cytochrome b5 reductase of bovine brain: purification and properties (1983), J. Biochem., 94, 1547-1555.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.6.2.2 inactivation during dialysis is prevented by NADH Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.2 Acrynol 0.1 mM, 75% inhibition Bos taurus
1.6.2.2 adenine nucleotides
-
 Bos taurus
1.6.2.2 Atebrin 1 mM, complete inhibition Bos taurus
1.6.2.2 Cl-
-
 Bos taurus
1.6.2.2 iodoacetic acid 5 mM, complete inhibition Bos taurus
1.6.2.2 N-ethylmaleimide 10 mM, 89% inhibition Bos taurus
1.6.2.2 p-chloromercuribenzoate 0.001 mM, complete inhibition Bos taurus
1.6.2.2 phosphate competitive inhibition Bos taurus
1.6.2.2 Proflavin 0.1 mM, 86% inhibition Bos taurus
1.6.2.2 taurodeoxycholate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.0011
-
 NADH
-
 Bos taurus
1.6.2.2 0.0042
-
 ferricyanide
-
 Bos taurus
1.6.2.2 0.0143
-
 ferricytochrome b5
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.2 endoplasmic reticulum
-
 Bos taurus 5783
-
1.6.2.2 membrane membrane-binding domain resides in the NH2-terminal region Bos taurus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.2 31000
-
 x * 31000, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2 lysosome-solubilization Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.2 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.2 137
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.6.2.2 0°C, at least 1 week Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricytochrome b5 + NADH specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol Bos taurus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH no acceptors: ubiquinone-30, menadione, dihydrofolate, lipoamide Bos taurus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH poor donor: NADPH Bos taurus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 NADH + ferricytochrome b5
-
 Bos taurus NAD+ + H+ + ferrocytochrome b5
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.2.2 ? x * 31000, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.2 7 7.5
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 NADH
-
 Bos taurus