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Literature summary extracted from

  • Iyanagi, T.; Watanabe, S.; Anan, K.F.
    One-electron oxidation-reduction properties of hepatic NADH-cytochrome b5 reductase (1984), Biochemistry, 23, 1418-1425.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.0004 0.0005 NADH lysosome- and detergent-solubilized enzyme Sus scrofa
1.6.2.2 0.03 0.04 cytochrome b5 lysosome- and detergent-solubilized enzyme Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.2 endoplasmic reticulum
-
 Sus scrofa 5783
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.6.2.2 additional information rate of reduction depends on ionic strength Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.2 30000
-
 x * 30000, lysosome-solubilized enzyme, SDS-PAGE Sus scrofa
1.6.2.2 35000
-
 x * 35000, detergent-solubilized enzyme, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2 lysosome- and detergent-solubilization Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
1.6.2.2 NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5 electron-transport chain from NADH to a terminal oxidase desaturase, proposed electron transfer mechanism Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.2 liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 NADH + ferricytochrome b5
-
 Sus scrofa NAD+ + H+ + ferrocytochrome b5
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.2.2 ? x * 30000, lysosome-solubilized enzyme, SDS-PAGE Sus scrofa
1.6.2.2 ? x * 35000, detergent-solubilized enzyme, SDS-PAGE Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 NADH
-
 Sus scrofa