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Literature summary extracted from

  • Shirabe, K.; Yubisui, T.; Takeshita, M.
    Expression of human erythrocyte NADH-cytochrome b5 reductase as an alpha-thrombin-cleavable fused protein in Escherichia coli (1989), Biochim. Biophys. Acta, 1008, 189-192.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.2 expression in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.0008
-
 NADH
-
 Homo sapiens
1.6.2.2 0.00114
-
 cytochrome b5
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.2 membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.2 33000
-
 recombinant enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.2 2 ferricytochrome b5 + NADH Homo sapiens soluble form of erythrocytes: reduction of methemoglobin 2 ferrocytochrome b5 + NAD+ + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.6.2.2 lipoprotein myristic acid at NH2-terminus, amide bond, no ester bond Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2 recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.2 erythrocyte soluble form Homo sapiens
-
1.6.2.2 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.2 96.3
-
 recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricytochrome b5 + NADH soluble form of erythrocytes: reduction of methemoglobin Homo sapiens 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 NADH + ferricytochrome b5
-
 Homo sapiens NAD+ + H+ + ferrocytochrome b5
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 FAD 1 mol FAD per mol of recombinant enzyme Homo sapiens
1.6.2.2 NADH
-
 Homo sapiens