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Literature summary extracted from

  • Bonomi, F.; Long, R.C.; Kurtz, D.M.
    Purification and properties of a membrane-bound NADH-cytochrome-b5 reductase from erythrocytes of the sipunculid worm, Phascolopsis gouldii (1989), Biochim. Biophys. Acta, 999, 147-156.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.6.2.2 dithiothreitol is essential for stability Phascolopsis gouldii
1.6.2.2 EDTA is essential for stability Phascolopsis gouldii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.2 additional information not inhibited by NAD+ and ferrocyanide Phascolopsis gouldii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.0028
-
 cytochrome b5
-
 Phascolopsis gouldii
1.6.2.2 0.06
-
 NADH
-
 Phascolopsis gouldii
1.6.2.2 0.09
-
 ferricyanide
-
 Phascolopsis gouldii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.2 membrane
-
 Phascolopsis gouldii 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.2 34000
-
 x * 34000, SDS-PAGE Phascolopsis gouldii
1.6.2.2 200000
-
 oligomeric aggregate of detergent-solubilized enzyme in aqueous media, gel filtration Phascolopsis gouldii

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Phascolopsis gouldii
-
 sipuncilid worm
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.6.2.2 O2, solubilized enzyme very sensitive to atmospheric oxygen Phascolopsis gouldii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2
-
 Phascolopsis gouldii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.2 erythrocyte soluble form Phascolopsis gouldii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.2 3.5
-
 low MW aggregate, nitro blue tetrazolium as electron acceptor Phascolopsis gouldii
1.6.2.2 10.6
-
 low MW aggregate, cytochrome b as electron acceptor Phascolopsis gouldii
1.6.2.2 138
-
 low MW aggregate, dichlorophenolindophenol as electron acceptor Phascolopsis gouldii
1.6.2.2 230.5
-
 high MW aggregate Phascolopsis gouldii
1.6.2.2 611.1
-
 low MW aggregate, ferricyanide as electron acceptor Phascolopsis gouldii

Storage Stability

EC Number Storage Stability Organism
1.6.2.2 liquid N2 Phascolopsis gouldii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 methemerythrin + NADH
-
 Phascolopsis gouldii deoxymethemerythrin + NAD+
-
 ?
1.6.2.2 NADH + ferricyanide
-
 Phascolopsis gouldii NAD+ + H+ + ferrocyanide
-
 r
1.6.2.2 NADH + ferricytochrome b5
-
 Phascolopsis gouldii NAD+ + H+ + ferrocytochrome b5
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.2.2 ? x * 34000, SDS-PAGE Phascolopsis gouldii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.2 5.5
-
 acceptor ferricyanide Phascolopsis gouldii
1.6.2.2 6 8 acceptor Phascolopsis gouldii cytochrome b5 Phascolopsis gouldii
1.6.2.2 8
-
 acceptor dichlorphenolindophenol Phascolopsis gouldii

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 FAD
-
 Phascolopsis gouldii
1.6.2.2 NADH
-
 Phascolopsis gouldii