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Literature summary extracted from

  • Lucas, K.; Boland, M.J.; Schubert, K.R.
    Uricase from soybean root nodules: purification, properties, and comparison with the enzyme from cowpea (1983), Arch. Biochem. Biophys., 226, 190-197.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.3 adenine weak Glycine max
1.7.3.3 allantoin weak Glycine max
1.7.3.3 glutamine weak Glycine max
1.7.3.3 NH4+ weak Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.3.3 0.01
-
 uric acid
-
 Glycine max
1.7.3.3 0.031
-
 O2
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.3.3 32000 35000 SDS-PAGE, gel filtration Glycine max
1.7.3.3 68000
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.3.3 urate + O2 + H2O Glycine max
-
 5-hydroxyisourate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.3 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.3.3
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.3.3 seed
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.3.3 6.85
-
-
 Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.3 urate + O2 + H2O
-
 Glycine max 5-hydroxyisourate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.3.3 dimer at pH 8.8 the enzyme associates to form a dimer of about 68000 Glycine max
1.7.3.3 monomer native enzyme at pH 7.5 is a monomer with MW 32000-35000 Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.3.3 9.5
-
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.3.3 8 10.2 pH 8.0: about 35% of activity maximum, pH 10.2: about 55% of activity maximum Glycine max