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Literature summary extracted from

  • Hall, M.R.; Berk, R.S.
    Thiosulfate oxidase from an Alcaligenes grown on mercaptosuccinate (1972), Can. J. Microbiol., 18, 235-245.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.2.2 EDTA 18% inhibition at 1 mM and 46% inhibition at 20 mM Alcaligenes sp.
1.8.2.2 HgCl2 100% inhibition at 1 mM Alcaligenes sp.
1.8.2.2 N-ethylmaleimide 78% inhibition at 1 mM Alcaligenes sp.
1.8.2.2 p-chloromercuribenzoate 90% inhibition at 1 mM Alcaligenes sp.
1.8.2.2 SO32- 62% inhibition at 3 mM Alcaligenes sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.2.2 90000
-
 gel filtration Alcaligenes sp.

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.2 Alcaligenes sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.2 using MnCl2 precipitation, treatment with calcium phosphate gel, and successive column chromatography on DEAE-Sephadex and Sephadex G-150 Alcaligenes sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.2.2 additional information
-
 specific activities in crude cell-free extracts of mercaptosuccinate-grown Alcaligenes under a variety of nutritional conditions Alcaligenes sp.
1.8.2.2 6010
-
-
 Alcaligenes sp.

Storage Stability

EC Number Storage Stability Organism
1.8.2.2 4°C, purified enzyme, 10% loss of activity after 24 h, completely inactive after 7 days Alcaligenes sp.
1.8.2.2 frozen, 0.05 M phosphate buffer, pH 6.2, stable for at least 90 days Alcaligenes sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.2 additional information NAD+, methylene blue, 2,6-dichlorophenol indophenol Alcaligenes sp. ?
-
 ?
1.8.2.2 additional information no activity with FAD Alcaligenes sp. ?
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor strictly specific for thiosulfate Alcaligenes sp. tetrathionate + reduced acceptor
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor ferricyanide or native cytochrome c act as electron acceptors Alcaligenes sp. tetrathionate + reduced acceptor
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor cytochrome c acts as electron acceptor Alcaligenes sp. tetrathionate + reduced acceptor
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor ferricyanide acts as electron acceptor Alcaligenes sp. tetrathionate + reduced acceptor
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.2.2 40
-
-
 Alcaligenes sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.8.2.2 22 48 about 50% of activity maximum at 22°C and 48°C Alcaligenes sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.2.2 25
-
 30 min, 7% loss of activity Alcaligenes sp.
1.8.2.2 45
-
 30 min, complete loss of activity Alcaligenes sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.2.2 5
-
 below, using ferricyanide as electron acceptor Alcaligenes sp.
1.8.2.2 7.2 7.6 cytochrome c as electron acceptor Alcaligenes sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.2.2 5 9
-
 Alcaligenes sp.