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Literature summary extracted from

  • Trudinger, P.A.
    Thiosulfate oxidation and cytochromes in Thiobacillus X (1961), Biochem. J., 78, 680-686.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.2.2 azide not inhibitory at 10 mM Thiobacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.2 Thiobacillus sp.
-
 X
-
1.8.2.2 Thiobacillus sp. X
-
 X
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.2.2 additional information
-
-
 Thiobacillus sp.

Storage Stability

EC Number Storage Stability Organism
1.8.2.2 -25°C, 0.02 M phosphate, 40% loss of activity after 2 months Thiobacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.2 thiosulfate + oxidized acceptor cytochrome c acts as electron acceptor Thiobacillus sp. tetrathionate + reduced acceptor
-
 ir
1.8.2.2 thiosulfate + oxidized acceptor cytochrome 557 becames reduced when mixed with thiosulfate, enzyme and a trace of cytochrome 553.5, the omission of either the enzyme or cytochrome 553.5 prevents this reduction Thiobacillus sp. tetrathionate + reduced acceptor
-
 ir
1.8.2.2 thiosulfate + oxidized acceptor mammalian cytochrome c acts as electron acceptor Thiobacillus sp. tetrathionate + reduced acceptor
-
 ir
1.8.2.2 thiosulfate + oxidized acceptor cytochrome c553.5 acts as electron acceptor Thiobacillus sp. tetrathionate + reduced acceptor
-
 ir
1.8.2.2 thiosulfate + oxidized acceptor ferricyanide acts as electron acceptor Thiobacillus sp. tetrathionate + reduced acceptor
-
 ir
1.8.2.2 thiosulfate + oxidized acceptor cytochrome c acts as electron acceptor Thiobacillus sp. X tetrathionate + reduced acceptor
-
 ir
1.8.2.2 thiosulfate + oxidized acceptor cytochrome 557 becames reduced when mixed with thiosulfate, enzyme and a trace of cytochrome 553.5, the omission of either the enzyme or cytochrome 553.5 prevents this reduction Thiobacillus sp. X tetrathionate + reduced acceptor
-
 ir
1.8.2.2 thiosulfate + oxidized acceptor mammalian cytochrome c acts as electron acceptor Thiobacillus sp. X tetrathionate + reduced acceptor
-
 ir
1.8.2.2 thiosulfate + oxidized acceptor cytochrome c553.5 acts as electron acceptor Thiobacillus sp. X tetrathionate + reduced acceptor
-
 ir
1.8.2.2 thiosulfate + oxidized acceptor ferricyanide acts as electron acceptor Thiobacillus sp. X tetrathionate + reduced acceptor
-
 ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.2.2 45
-
 3 min, pH 7, complete loss of activity Thiobacillus sp.
1.8.2.2 60
-
 enzyme activity completely destroyed after 3 min at pH 7 Thiobacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.2.2 5
-
 the rate of reduction of cytochrome 553.5 at pH 5 is about 10% of the rate at pH 7 Thiobacillus sp.
1.8.2.2 7
-
 for the reduction of cytochrome 553.5 Thiobacillus sp.