EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.8.2.1 | phosphate | maximal actiovity at final substrate concentrations of 0.02-0.05 M at pH 7.8 | Rhodotorula sp. |
EC Number | General Stability | Organism |
---|---|---|
1.8.2.1 | treatment with 1-2 mg trypsin per 1.5 mg of protein or 0.1-0.2 mg of pronase or 1.5 mg of protein at 25°C causes decline of activity | Rhodotorula sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.8.2.1 | 1,10-phenanthroline | 5 mM, 30% inhibition | Rhodotorula sp. | |
1.8.2.1 | 2,2'-bipyridyl | 5 mM, 50% inhibition | Rhodotorula sp. | |
1.8.2.1 | AMP | 0.01 mM, 50% inhibition | Rhodotorula sp. | |
1.8.2.1 | EDTA | 10 mM Na2EDTA, 30% inhibition | Rhodotorula sp. | |
1.8.2.1 | HgCl2 | 0.25 mM, complete inhibition | Rhodotorula sp. | |
1.8.2.1 | NEM | 1 mM, complete inhibition | Rhodotorula sp. | |
1.8.2.1 | p-hydroxymercuribenzoate | 1 mM, complete inhibition | Rhodotorula sp. | |
1.8.2.1 | sodium deoxycholate | 1.0%, 80% inhibition | Rhodotorula sp. | |
1.8.2.1 | Triton X-100 | 1.0%, 15% inhibition | Rhodotorula sp. | |
1.8.2.2 | 1,10-phenanthroline | 17% inhibition at 5 mM | Rhodotorula sp. | |
1.8.2.2 | 2,2'-bipyridyl | not inhibitory at 5 mM | Rhodotorula sp. | |
1.8.2.2 | deoxycholate | - |
Rhodotorula sp. | |
1.8.2.2 | HgCl2 | 100% inhibition at 0.5 mM, 45% inhibition at 0.25 mM and 15% inhibition at 0.1 mM | Rhodotorula sp. | |
1.8.2.2 | N-ethylmaleimide | 12% inhibition at 1 mM | Rhodotorula sp. | |
1.8.2.2 | p-hydroxymercuribenzoate | 50% inhibition at 1 mM, 40% inhibition at 0.5 mM and 10% inhibition at 0.1 mM | Rhodotorula sp. | |
1.8.2.2 | SO32- | - |
Rhodotorula sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.2.1 | 0.06 | - |
sulfite | reaction with cytochrome c | Rhodotorula sp. | |
1.8.2.1 | 0.42 | - |
ferricyanide | - |
Rhodotorula sp. | |
1.8.2.1 | 1 | - |
sulfite | reaction with ferricyanide | Rhodotorula sp. | |
1.8.2.2 | 0.14 | - |
thiosulfate | with native cytochrome c as electron acceptor | Rhodotorula sp. | |
1.8.2.2 | 0.16 | - |
thiosulfate | with ferricyanide as electron acceptor | Rhodotorula sp. | |
1.8.2.2 | 0.34 | - |
ferricyanide | - |
Rhodotorula sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.2.1 | Rhodotorula sp. | - |
enzyme complex that posseses sulfite oxidase activity and thiosulfate-oxidizing activity | - |
1.8.2.2 | Rhodotorula sp. | - |
- |
- |
EC Number | Storage Stability | Organism |
---|---|---|
1.8.2.1 | -20°C, in presence of 20% glycerol stable for several days, without glycerol most of the activity is lost after 48 h | Rhodotorula sp. |
1.8.2.2 | -20°C, the activity of cell-free extracts is stable for several days in the presence of 20% v/v glycerol, without glycerol most of the activity is lost after 2 days | Rhodotorula sp. |
1.8.2.2 | -20°C, the activity of whole cells is stable for 3 months | Rhodotorula sp. |
1.8.2.2 | 4°C, cell-free extracts lose most of the activity after 2 days even in the presence of glycerol | Rhodotorula sp. |
1.8.2.2 | the activity in cell-free extracts is lost very rapidly after freezing and thawing | Rhodotorula sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.2.1 | sulfite + ferricyanide + H2O | - |
Rhodotorula sp. | sulfate + ferrocyanide + H+ | - |
? | |
1.8.2.1 | sulfite + ferricytochrome c + H2O | - |
Rhodotorula sp. | sulfate + ferrocytochrome c + H+ | - |
? | |
1.8.2.2 | thiosulfate + ferricyanide | - |
Rhodotorula sp. | tetrathionate + ferrocyanide | - |
? | |
1.8.2.2 | thiosulfate + oxidized acceptor | ferricyanide or native cytochrome c act as electron acceptors | Rhodotorula sp. | tetrathionate + reduced acceptor | - |
? | |
1.8.2.2 | thiosulfate + oxidized acceptor | cytochrome c acts as electron acceptor | Rhodotorula sp. | tetrathionate + reduced acceptor | - |
? | |
1.8.2.2 | thiosulfate + oxidized acceptor | the activity does not couple with mammalian cytochrome c as electron acceptor | Rhodotorula sp. | tetrathionate + reduced acceptor | - |
? | |
1.8.2.2 | thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Rhodotorula sp. | tetrathionate + reduced acceptor | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.2.1 | 30 | - |
- |
Rhodotorula sp. |
1.8.2.2 | 30 | - |
- |
Rhodotorula sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.2.1 | 55 | - |
5 min, 60% loss of activity | Rhodotorula sp. |
1.8.2.1 | 60 | - |
7 min, 70% loss of activity | Rhodotorula sp. |
1.8.2.1 | 100 | - |
boiling of the purified enzyme complex destroys activity completely | Rhodotorula sp. |
1.8.2.2 | 55 | - |
30% decrease of activity after 5 min | Rhodotorula sp. |
1.8.2.2 | 60 | - |
50% decrease of activity after 7 min | Rhodotorula sp. |
1.8.2.2 | 100 | - |
after 2 min the activity is completely destroyed | Rhodotorula sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.2.1 | 7.8 | - |
reaction with native cytochrome c | Rhodotorula sp. |
1.8.2.2 | 7.8 | - |
- |
Rhodotorula sp. |