Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kurek, E.J.
    Properties of an enzymatic complex active in sulfite and thiosulfate oxidation by Rhodotorula sp. (1985), Arch. Microbiol., 143, 277-282.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.2.1 phosphate maximal actiovity at final substrate concentrations of 0.02-0.05 M at pH 7.8 Rhodotorula sp.

General Stability

EC Number General Stability Organism
1.8.2.1 treatment with 1-2 mg trypsin per 1.5 mg of protein or 0.1-0.2 mg of pronase or 1.5 mg of protein at 25°C causes decline of activity Rhodotorula sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.2.1 1,10-phenanthroline 5 mM, 30% inhibition Rhodotorula sp.
1.8.2.1 2,2'-bipyridyl 5 mM, 50% inhibition Rhodotorula sp.
1.8.2.1 AMP 0.01 mM, 50% inhibition Rhodotorula sp.
1.8.2.1 EDTA 10 mM Na2EDTA, 30% inhibition Rhodotorula sp.
1.8.2.1 HgCl2 0.25 mM, complete inhibition Rhodotorula sp.
1.8.2.1 NEM 1 mM, complete inhibition Rhodotorula sp.
1.8.2.1 p-hydroxymercuribenzoate 1 mM, complete inhibition Rhodotorula sp.
1.8.2.1 sodium deoxycholate 1.0%, 80% inhibition Rhodotorula sp.
1.8.2.1 Triton X-100 1.0%, 15% inhibition Rhodotorula sp.
1.8.2.2 1,10-phenanthroline 17% inhibition at 5 mM Rhodotorula sp.
1.8.2.2 2,2'-bipyridyl not inhibitory at 5 mM Rhodotorula sp.
1.8.2.2 deoxycholate
-
Rhodotorula sp.
1.8.2.2 HgCl2 100% inhibition at 0.5 mM, 45% inhibition at 0.25 mM and 15% inhibition at 0.1 mM Rhodotorula sp.
1.8.2.2 N-ethylmaleimide 12% inhibition at 1 mM Rhodotorula sp.
1.8.2.2 p-hydroxymercuribenzoate 50% inhibition at 1 mM, 40% inhibition at 0.5 mM and 10% inhibition at 0.1 mM Rhodotorula sp.
1.8.2.2 SO32-
-
Rhodotorula sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.1 0.06
-
sulfite reaction with cytochrome c Rhodotorula sp.
1.8.2.1 0.42
-
ferricyanide
-
Rhodotorula sp.
1.8.2.1 1
-
sulfite reaction with ferricyanide Rhodotorula sp.
1.8.2.2 0.14
-
thiosulfate with native cytochrome c as electron acceptor Rhodotorula sp.
1.8.2.2 0.16
-
thiosulfate with ferricyanide as electron acceptor Rhodotorula sp.
1.8.2.2 0.34
-
ferricyanide
-
Rhodotorula sp.

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.1 Rhodotorula sp.
-
enzyme complex that posseses sulfite oxidase activity and thiosulfate-oxidizing activity
-
1.8.2.2 Rhodotorula sp.
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.8.2.1 -20°C, in presence of 20% glycerol stable for several days, without glycerol most of the activity is lost after 48 h Rhodotorula sp.
1.8.2.2 -20°C, the activity of cell-free extracts is stable for several days in the presence of 20% v/v glycerol, without glycerol most of the activity is lost after 2 days Rhodotorula sp.
1.8.2.2 -20°C, the activity of whole cells is stable for 3 months Rhodotorula sp.
1.8.2.2 4°C, cell-free extracts lose most of the activity after 2 days even in the presence of glycerol Rhodotorula sp.
1.8.2.2 the activity in cell-free extracts is lost very rapidly after freezing and thawing Rhodotorula sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.1 sulfite + ferricyanide + H2O
-
Rhodotorula sp. sulfate + ferrocyanide + H+
-
?
1.8.2.1 sulfite + ferricytochrome c + H2O
-
Rhodotorula sp. sulfate + ferrocytochrome c + H+
-
?
1.8.2.2 thiosulfate + ferricyanide
-
Rhodotorula sp. tetrathionate + ferrocyanide
-
?
1.8.2.2 thiosulfate + oxidized acceptor ferricyanide or native cytochrome c act as electron acceptors Rhodotorula sp. tetrathionate + reduced acceptor
-
?
1.8.2.2 thiosulfate + oxidized acceptor cytochrome c acts as electron acceptor Rhodotorula sp. tetrathionate + reduced acceptor
-
?
1.8.2.2 thiosulfate + oxidized acceptor the activity does not couple with mammalian cytochrome c as electron acceptor Rhodotorula sp. tetrathionate + reduced acceptor
-
?
1.8.2.2 thiosulfate + oxidized acceptor ferricyanide acts as electron acceptor Rhodotorula sp. tetrathionate + reduced acceptor
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.2.1 30
-
-
Rhodotorula sp.
1.8.2.2 30
-
-
Rhodotorula sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.2.1 55
-
5 min, 60% loss of activity Rhodotorula sp.
1.8.2.1 60
-
7 min, 70% loss of activity Rhodotorula sp.
1.8.2.1 100
-
boiling of the purified enzyme complex destroys activity completely Rhodotorula sp.
1.8.2.2 55
-
30% decrease of activity after 5 min Rhodotorula sp.
1.8.2.2 60
-
50% decrease of activity after 7 min Rhodotorula sp.
1.8.2.2 100
-
after 2 min the activity is completely destroyed Rhodotorula sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.2.1 7.8
-
reaction with native cytochrome c Rhodotorula sp.
1.8.2.2 7.8
-
-
Rhodotorula sp.