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Literature summary extracted from
- Desulfoviridin, a multimeric-dissimilatory sulfite reductase from Desulfovibrio vulgaris (Hildenborough). Purification, characterization, kinetics and EPR studies (1994), Eur. J. Biochem., 223, 79-89.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.99.5 | 0.028 | - |
nitrite | pH 7.6, 22°C | Desulfovibrio vulgaris |  |
| 1.8.99.5 | 0.06 | - |
sulfite | pH 7.6, 22°C | Desulfovibrio vulgaris | |
| 1.8.99.5 | 48 | - |
hydroxylamine | pH 7.6, 22°C | Desulfovibrio vulgaris | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.99.5 | Desulfovibrio vulgaris | - |
- |
- |
| 1.8.99.5 | Desulfovibrio vulgaris Hildenborough | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.99.5 | - |
Desulfovibrio vulgaris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.99.5 | hydroxylamine + a [DsrC protein]-dithiol + 2 reduced acceptor + 2 H+ | - |
Desulfovibrio vulgaris | ? + a [DsrC protein]-disulfide + 2 acceptor + 3 H2O | - |
? | |
| 1.8.99.5 | hydroxylamine + a [DsrC protein]-dithiol + 2 reduced acceptor + 2 H+ | - |
Desulfovibrio vulgaris Hildenborough | ? + a [DsrC protein]-disulfide + 2 acceptor + 3 H2O | - |
? | |
| 1.8.99.5 | nitrite + a [DsrC protein]-dithiol + 2 reduced acceptor | - |
Desulfovibrio vulgaris | ? + a [DsrC protein]-disulfide + 2 acceptor + 2 H2O | - |
? | |
| 1.8.99.5 | nitrite + a [DsrC protein]-dithiol + 2 reduced acceptor | - |
Desulfovibrio vulgaris Hildenborough | ? + a [DsrC protein]-disulfide + 2 acceptor + 2 H2O | - |
? | |
| 1.8.99.5 | sulfite + a [DsrC protein]-dithiol + 2 reduced ferredoxin + 2 H+ | - |
Desulfovibrio vulgaris | hydrogen sulfide + a [DsrC protein]-disulfide + 2 oxidized ferredoxin + 3 H2O | overall reaction | ? | |
| 1.8.99.5 | sulfite + a [DsrC protein]-dithiol + 2 reduced ferredoxin + 2 H+ | - |
Desulfovibrio vulgaris Hildenborough | hydrogen sulfide + a [DsrC protein]-disulfide + 2 oxidized ferredoxin + 3 H2O | overall reaction | ? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.99.5 | 0.038 | - |
nitrite | pH 7.6, 22°C | Desulfovibrio vulgaris | |
| 1.8.99.5 | 0.31 | - |
sulfite | pH 7.6, 22°C | Desulfovibrio vulgaris | |
| 1.8.99.5 | 29 | - |
hydroxylamine | pH 7.6, 22°C | Desulfovibrio vulgaris | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.5 | siroheme | at least two of three subunits contain two pairs of [Fe4-S4]-centers and siroheme units | Desulfovibrio vulgaris | |
| 1.8.99.5 | [4Fe-4S]-center | at least two of three subunits contain two pairs of [Fe4-S4]-centers and siroheme units | Desulfovibrio vulgaris | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.99.5 | 0.6 | - |
hydroxylamine | pH 7.6, 22°C | Desulfovibrio vulgaris | |
| 1.8.99.5 | 1.4 | - |
nitrite | pH 7.6, 22°C | Desulfovibrio vulgaris | |
| 1.8.99.5 | 5.17 | - |
sulfite | pH 7.6, 22°C | Desulfovibrio vulgaris | |
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