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Literature summary extracted from

  • Shimaoka, T.; Yokota, A.; Miyake, C.
    Purification and characterization of chloroplast dehydroascorbate reductase from spinach leaves (2000), Plant Cell Physiol., 41, 1110-1118.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.5.1 enzyme form DHAR-a, expression in Escherichia coli Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.5.1 iodoacetic acid
-
 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.1 0.06
-
 dehydroascorbate recombinant enzyme form DHAR-1 Spinacia oleracea
1.8.5.1 0.07
-
 dehydroascorbate enzyme form DHAR-a Spinacia oleracea
1.8.5.1 0.08
-
 dehydroascorbate enzyme form DHAR-b Spinacia oleracea
1.8.5.1 1.1
-
 GSH enzyme form DHAR-a and recombinant enzyme form DHAR-a Spinacia oleracea
1.8.5.1 2.5
-
 GSH enzyme form DHAR-b Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.5.1 chloroplast stroma Spinacia oleracea 9507
-
1.8.5.1 additional information two major dehydroascorbate reductases exist in spinach leaves. One form, DHAR-1, originates in chloroplast, the other, DHAR-b, occurs in a subcellular compartment other than chloroplast Spinacia oleracea
-
-
1.8.5.1 plastid
-
 Spinacia oleracea 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.5.1 25000
-
 1 * 25000, enzyme form DHAR-b, SDS-PAGE Spinacia oleracea
1.8.5.1 26000
-
 1 * 26000, enzyme form DHAR-a, SDS-PAGE Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.1 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.1
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.5.1 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.5.1 360
-
 mitochondrial enzyme Spinacia oleracea
1.8.5.1 400
-
 plastid enzyme Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
1.8.5.1 4°C, stable for at least 1 week Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.1 GSH + dehydroascorbate
-
 Spinacia oleracea GSSG + ascorbate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.5.1 monomer 1 * 25000, enzyme form DHAR-b, SDS-PAGE Spinacia oleracea
1.8.5.1 monomer 1 * 26000, enzyme form DHAR-a, SDS-PAGE Spinacia oleracea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.5.1 80
-
 10 min, 81% loss of activity of enzyme form DHAR-a, 97% loss of activity of enzyme form DHAR-b Spinacia oleracea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.5.1 additional information
-
 additional information
-
 Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.5.1 7.7
-
 enzyme form DHAR-b Spinacia oleracea
1.8.5.1 7.9
-
 enzyme form DHAR-a Spinacia oleracea