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Literature summary extracted from

  • Kim, Y.R.; Kang, S.O.
    Purification and characterization of dehydroascorbate reductase from Pleurotus ostreatus (1998), J. Microbiol., 36, 164-170.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.5.1 Ca2+ 5 mM, 34% loss of activity Pleurotus ostreatus
1.8.5.1 Cd2+ 5 mM CdCl2, complete loss of activity Pleurotus ostreatus
1.8.5.1 Cu2+ 0.1 mM CuSO4, 43% loss of activity Pleurotus ostreatus
1.8.5.1 Fe2+ 0.1 mM FeSO4, 34% loss of activity Pleurotus ostreatus
1.8.5.1 HgCl2 5 mM HgCl2, 94% loss of activity Pleurotus ostreatus
1.8.5.1 imidazole 1 mM, 14% loss of activity Pleurotus ostreatus
1.8.5.1 iodoacetic acid 1 mM, 26% loss of activity Pleurotus ostreatus
1.8.5.1 Mn2+ 5 mM MnSO4, 82% loss of activity Pleurotus ostreatus
1.8.5.1 NEM 1 mM, 23% loss of activity Pleurotus ostreatus
1.8.5.1 PCMB 1 mM, 32% loss of activity Pleurotus ostreatus
1.8.5.1 Sodium azide 1 mM, 32% loss of activity Pleurotus ostreatus
1.8.5.1 Zn2+ 5 mM ZnSO4, 50% loss of activity Pleurotus ostreatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.1 0.7
-
 GSH
-
 Pleurotus ostreatus
1.8.5.1 2.5
-
 dehydroascorbate
-
 Pleurotus ostreatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.5.1 cytosol
-
 Pleurotus ostreatus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.5.1 86000
-
 gel filtration Pleurotus ostreatus

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.1 Pleurotus ostreatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.1
-
 Pleurotus ostreatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.5.1 mycelium
-
 Pleurotus ostreatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.5.1 9.3
-
-
 Pleurotus ostreatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.1 GSH + dehydroascorbate specific for glutathione as hydrogen donor Pleurotus ostreatus GSSG + ascorbate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.5.1 monomer 1 * 86000, SDS-PAGE Pleurotus ostreatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.5.1 38 45
-
 Pleurotus ostreatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.5.1 50
-
 pH 6.2, 1 h, stable below Pleurotus ostreatus
1.8.5.1 65
-
 complete loss of activity above Pleurotus ostreatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.5.1 7.5
-
-
 Pleurotus ostreatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.5.1 6.5 8.5 pH 6.5: about 25% of maximal activity, pH 8.5: about 60% of maximal activity Pleurotus ostreatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.8.5.1 6.5
-
 highest stability at, unstable under acidic and highly alkaline conditions Pleurotus ostreatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.5.1 flavin enzyme shows an unusual flavin peak, enzyme might form a flavin adduct Pleurotus ostreatus