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Literature summary extracted from

  • Shigeoka, S.; Yasumoto, R.; Onishi, T.; Nakano, Y.; Kitaoka, S.
    Properties of monodehydroascorbate reductase and dehydroascorbate reductase and their participation in the regeneration of ascorbate in Euglena gracilis (1987), J. Gen. Microbiol., 133, 227-232.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.5.1 2-mercaptoethanol 1 mM, stimulates about 2.1fold Euglena gracilis
1.8.5.1 dithiothreitol 1 mM, stimulates about 2.7fold Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.5.1 Co2+
-
 Euglena gracilis
1.8.5.1 Fe3+ 1 mM, complete inhibition Euglena gracilis
1.8.5.1 NEM 0.5 mM, reversed by thiol reagents Euglena gracilis
1.8.5.1 PCMB 0.05 mM, reversed by thiol reagents Euglena gracilis
1.8.5.1 Zn2+ 1 mM, complete inhibition Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.1 0.26
-
 dehydroascorbate
-
 Euglena gracilis
1.8.5.1 0.85
-
 glutathione
-
 Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.5.1 cytosol
-
 Euglena gracilis 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.5.1 28000
-
 gel filtration Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.1 Euglena gracilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.1 partial Euglena gracilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.5.1 7.68
-
-
 Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.1 GSH + dehydroascorbate
-
 Euglena gracilis GSSG + ascorbate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.5.1 38
-
-
 Euglena gracilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.5.1 42
-
 pH 7.0-8.0, stable up to Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.5.1 7
-
-
 Euglena gracilis