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Literature summary extracted from

  • Bramlett, R.N.; Peck, H.D.
    Some physical and kinetic properties of adenylyl sulfate reductase from Desulfovibrio vulgaris (1975), J. Biol. Chem., 250, 2979-2986.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.99.2 0.019
-
 adenylyl sulfate
-
 Desulfovibrio vulgaris
1.8.99.2 0.025
-
 guanylyl sulfate
-
 Desulfovibrio vulgaris
1.8.99.2 0.18
-
 AMP reaction with cytochrome c Desulfovibrio vulgaris
1.8.99.2 0.3
-
 AMP reaction with ferricyanide Desulfovibrio vulgaris
1.8.99.2 0.8
-
 IMP reaction with ferricyanide Desulfovibrio vulgaris
1.8.99.2 1.25
-
 GMP reaction with cytochrome c Desulfovibrio vulgaris
1.8.99.2 1.5
-
 IMP reaction with cytochrome c Desulfovibrio vulgaris
1.8.99.2 3
-
 GMP reaction with ferricyanide Desulfovibrio vulgaris
1.8.99.2 3.1
-
 CMP reaction with cytochrome c Desulfovibrio vulgaris
1.8.99.2 4.5
-
 UMP reaction with cytochrome c Desulfovibrio vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.99.2 Iron enzyme contains 12.7 mol of iron per 22000 Da enzyme Desulfovibrio vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.99.2 20000
-
 x * 72000 + x * 20000, SDS-PAGE Desulfovibrio vulgaris
1.8.99.2 72000
-
 x * 72000 + x * 20000, SDS-PAGE Desulfovibrio vulgaris
1.8.99.2 439500
-
 equilibrium sedimentation Desulfovibrio vulgaris

Organism

EC Number Organism UniProt Comment Textmining
1.8.99.2 Desulfovibrio vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.99.2
-
 Desulfovibrio vulgaris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.99.2 5.75
-
-
 Desulfovibrio vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.99.2 adenylyl sulfate + reduced methyl viologen
-
 Desulfovibrio vulgaris AMP + sulfite + methyl viologen
-
 ?
1.8.99.2 AMP + sulfite + cytochrome c
-
 Desulfovibrio vulgaris adenylyl sulfate + ?
-
 ?
1.8.99.2 CMP + sulfite + ferricyanide
-
 Desulfovibrio vulgaris cytidylyl sulfate + ferrocyanide
-
 ?
1.8.99.2 GMP + sulfite + ferricyanide
-
 Desulfovibrio vulgaris guanylyl sulfate + ferrocyanide
-
 ?
1.8.99.2 guanylyl sulfate + reduced methyl viologen
-
 Desulfovibrio vulgaris GMP + methyl viologen + sulfite
-
 ?
1.8.99.2 IMP + sulfite + ferricyanide
-
 Desulfovibrio vulgaris ? + ferrocyanide
-
 ?
1.8.99.2 UMP + sulfite + ferricyanide
-
 Desulfovibrio vulgaris uridylyl sulfate + ferrocyanide
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.99.2 ? x * 72000 + x * 20000, SDS-PAGE Desulfovibrio vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.99.2 7 7.5 oxidation of methyl viologen Desulfovibrio vulgaris
1.8.99.2 7.5
-
 reaction with cytochrome c and UMP or CMP Desulfovibrio vulgaris
1.8.99.2 9
-
 reaction with cytochrome c and IMP or GMP Desulfovibrio vulgaris
1.8.99.2 9.5
-
 reaction with cytochrome c and AMP Desulfovibrio vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.99.2 FAD enzyme contains 1 mol of FAD per mol of enzyme Desulfovibrio vulgaris