Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fujita, Y.; Bauer, C.E.
    Reconstitution of light-independent protochlorophyllide reductase from purified bchl and BchN-BchB subunits. In vitro confirmation of nitrogenase-like features of a bacteriochlorophyll biosynthesis enzyme (2000), J. Biol. Chem., 275, 23583-23588.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.7.7 Dithionite dark protochlorophyllide reductase activity is dependent on the reductant dithionite Rhodobacter capsulatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.7.7 subunits BchL and BchN are expressed in Rhodobacter capsulatus strains YCN1 or YCL3 as S tag fusion proteins, subunit BchB is co-purified with the BchN protein Rhodobacter capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.7.7 31000
-
 2 * 31000, L-protein of DPOR, SDS-PAGE Rhodobacter capsulatus
1.3.7.7 33413
-
 2 * 33413, L-protein of DPOR, calculated from amino acid sequence Rhodobacter capsulatus
1.3.7.7 43000
-
 2 * 51000 + 2 * 43000 , NB-protein of DPOR, SDS-PAGE Rhodobacter capsulatus
1.3.7.7 46038
-
 2 * 57191 + 2 * 46038, NB-protein of DPOR, calculated from amino acid sequence Rhodobacter capsulatus
1.3.7.7 51000
-
 2 * 51000 + 2 * 43000 , NB-protein of DPOR, SDS-PAGE Rhodobacter capsulatus
1.3.7.7 57191
-
 2 * 57191 + 2 * 46038, NB-protein of DPOR, calculated from amino acid sequence Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH Chlamydomonas reinhardtii
-
 chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH Rhodobacter capsulatus
-
 chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH Leptolyngbya boryana
-
 chlorophyllide + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.33 Chlamydomonas reinhardtii
-
-
-
1.3.1.33 Leptolyngbya boryana
-
 cyanobacterium
-
1.3.1.33 Rhodobacter capsulatus
-
-
-
1.3.7.7 Rhodobacter capsulatus
-
 strain strain CB1029
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.7.7 S protein-agarose column chromatography Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH
-
 Chlamydomonas reinhardtii chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH
-
 Rhodobacter capsulatus chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH
-
 Leptolyngbya boryana chlorophyllide + NADP+
-
 ?
1.3.7.7 protochlorophyllide + oxidized ferredoxin + ADP + phosphate
-
 Rhodobacter capsulatus chlorophyllide a + reduced ferredoxin + ATP
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.7.7 heterotetramer 2 * 51000 + 2 * 43000 , NB-protein of DPOR, SDS-PAGE Rhodobacter capsulatus
1.3.7.7 heterotetramer 2 * 57191 + 2 * 46038, NB-protein of DPOR, calculated from amino acid sequence Rhodobacter capsulatus
1.3.7.7 homodimer 2 * 31000, L-protein of DPOR, SDS-PAGE Rhodobacter capsulatus
1.3.7.7 homodimer 2 * 33413, L-protein of DPOR, calculated from amino acid sequence Rhodobacter capsulatus

Synonyms

EC Number Synonyms Comment Organism
1.3.7.7 dark protochlorophyllide reductase
-
 Rhodobacter capsulatus
1.3.7.7 DPOR
-
 Rhodobacter capsulatus
1.3.7.7 light-independent protochlorophyllide reductase
-
 Rhodobacter capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.7.7 ADP
-
 Rhodobacter capsulatus