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Literature summary extracted from

  • Safrany, S.T.; Caffrey, J.J.; Yang, X.; Bembenek, M.E.; Moyer, M.B.; Burkhart, W.A.; Shears, S.B.
    A novel context for the 'MutT' module, a guardian of cell integrity, in a diphosphoinositol polyphosphate phosphohydrolase (1998), EMBO J., 17, 6599-6607.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.52 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.52 E70Q
-
 Homo sapiens
3.6.1.52 E70Q greatly reduced rate of hydrolysis of diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.52 F- uncompetitive against bisdiphosphoinositol tetrakisphosphate, non-competitive against diphosphoinositol pentakisphosphate Rattus norvegicus
3.6.1.52 Inositol hexakisphosphate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.52 0.000034
-
 bisdiphosphoinositol tetrakisphosphate
-
 Rattus norvegicus
3.6.1.52 0.00034
-
 diphosphoinositol pentakisphosphate
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.52 Mg2+ 1-2 mM required Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.52 18000
-
 gel filtration Rattus norvegicus
3.6.1.52 19500
-
 x * 19500, calculation from nucleotide sequence Homo sapiens
3.6.1.52 25000
-
 x * 25000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.52 Homo sapiens
-
 recombinant enzyme
-
3.6.1.52 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.52
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.52 liver
-
 Rattus norvegicus
-
3.6.1.52 uterus
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.52 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.52 bisdiphosphoinositol tetrakisphosphate + H2O
-
 Homo sapiens ? + phosphate
-
 ?
3.6.1.52 bisdiphosphoinositol tetrakisphosphate + H2O
-
 Rattus norvegicus ? + phosphate
-
 ?
3.6.1.52 dATP + H2O
-
 Homo sapiens dADP + phosphate
-
 ?
3.6.1.52 diphosphoinositol pentakisphosphate + H2O
-
 Rattus norvegicus inositol hexakisphosphate + phosphate
-
 ?
3.6.1.52 diphosphoinositol pentakisphosphate + H2O the diphosphate group that is attached to the 5-carbon is hydrolyzed, no release of diphosphate Homo sapiens inositol hexakisphosphate + phosphate
-
 ?
3.6.1.52 additional information no activity with ATP Rattus norvegicus ?
-
 ?
3.6.1.52 additional information the MuT domain of the enzyme participates in the hydrolysis of dATP, diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate Homo sapiens ?
-
 ?
3.6.1.52 additional information the MuT domain of the enzyme participates in the hydrolysis of dATP, diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.52 ? x * 19500, calculation from nucleotide sequence Homo sapiens
3.6.1.52 ? x * 25000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.52 7 7.5 hydrolysis of diphosphoinositol pentakisphosphate Homo sapiens
3.6.1.52 9
-
 hydrolysis of dATP Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.52 6.5 8 pH 6.5: about 55% of maximal activity, pH 8.0: about 65% of maximal activity, hydrolysis of diphosphoinositol pentakisphosphate Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.52 0.0084
-
 F- against bisdiphosphoinositol tetrakisphosphate Rattus norvegicus
3.6.1.52 0.0109
-
 F- against diphosphoinositol pentakisphosphate Rattus norvegicus