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Literature summary extracted from

  • Giglione, C.; Pierre, M.; Meinnel, T.
    Peptide deformylase as a target for new generation, broad spectrum antimicrobial agents (2000), Mol. Microbiol., 36, 1197-1205.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.88 medicine possible target for new broad spectrum antimicrobial agents Staphylococcus aureus
3.5.1.88 medicine possible target for new broad spectrum antimicrobial agents Escherichia coli
3.5.1.88 medicine possible target for new broad spectrum antimicrobial agents Geobacillus stearothermophilus
3.5.1.88 medicine possible target for new broad spectrum antimicrobial agents Enterococcus faecalis
3.5.1.88 medicine possible target for new broad spectrum antimicrobial agents Plasmodium falciparum
3.5.1.88 medicine possible target for new broad spectrum antimicrobial agents Trypanosoma sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.88 additional information construction of a C-terminal 21 amino acid truncated enzyme Escherichia coli

General Stability

EC Number General Stability Organism
3.5.1.88 enhanced in vitro stability by binding of Zn2+ Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.88 actinonin
-
 Arabidopsis thaliana
3.5.1.88 actinonin pseudo-peptide hydroxamate, potent inhibitor Kd: 0.3 nM Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.88 Fe2+ sensitive to reactive oxygen Escherichia coli
3.5.1.88 Fe3+ more stable activity than with Fe2+ Escherichia coli
3.5.1.88 Ni2+ gives insensitivity to oxidation Escherichia coli
3.5.1.88 Zn2+ cruical for activity Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O Arabidopsis thaliana
-
 formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O Plasmodium falciparum
-
 formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O Trypanosoma sp.
-
 formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O Staphylococcus aureus deformylation required for methionine aminopeptidase activity in eubacteria, highly specific formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O Escherichia coli deformylation required for methionine aminopeptidase activity in eubacteria, highly specific formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O Geobacillus stearothermophilus deformylation required for methionine aminopeptidase activity in eubacteria, highly specific formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O Enterococcus faecalis deformylation required for methionine aminopeptidase activity in eubacteria, highly specific formate + L-methionine-polypeptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.88 Arabidopsis thaliana
-
-
-
3.5.1.88 Enterococcus faecalis
-
-
-
3.5.1.88 Escherichia coli
-
-
-
3.5.1.88 Geobacillus stearothermophilus
-
-
-
3.5.1.88 no activity in Caenorhabditis elegans
-
-
-
3.5.1.88 no activity in Saccharomyces cerevisiae
-
-
-
3.5.1.88 Plasmodium falciparum
-
-
-
3.5.1.88 Solanum lycopersicum
-
-
-
3.5.1.88 Staphylococcus aureus
-
-
-
3.5.1.88 Trypanosoma sp.
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.5.1.88 sensitive to intracellular reactive oxygen Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O
-
 Arabidopsis thaliana formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O
-
 Plasmodium falciparum formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O
-
 Trypanosoma sp. formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O deformylation required for methionine aminopeptidase activity in eubacteria, highly specific Staphylococcus aureus formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O deformylation required for methionine aminopeptidase activity in eubacteria, highly specific Escherichia coli formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O deformylation required for methionine aminopeptidase activity in eubacteria, highly specific Geobacillus stearothermophilus formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O deformylation required for methionine aminopeptidase activity in eubacteria, highly specific Enterococcus faecalis formate + L-methionine-polypeptide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.88 monomer
-
 Escherichia coli