EC Number | General Stability | Organism |
---|---|---|
3.2.1.52 | stable to repeated freeze-thaw cycles and to lyophilization, 20-30% of activity are lost if enzyme concentration is decreased to 0.02 mg/ml, stable with 0.1 mg bovine serum albumin per ml | Streptomyces sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.52 | 0.23 | - |
(N-acetyl-beta-D-glucosamine)3 | - |
Streptomyces sp. | |
3.2.1.52 | 0.7 | - |
dansyl-Asn(GlcNAc)2Man | - |
Streptomyces sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.52 | extracellular | - |
Streptomyces sp. | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 46000 | - |
gel filtration | Streptomyces sp. |
3.2.1.52 | 49500 | - |
sedimentation equilibrium | Streptomyces sp. |
3.2.1.52 | 52000 | - |
SDS-PAGE | Streptomyces sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.52 | N-acetyl-beta-D-hexosaminides + H2O | Streptomyces sp. | - |
N-acetyl-D-hexosamines | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.2.1.52 | sodium dodecyl sulfate | complete inactivation with 0.2% in less than 2 h at 37°C | Streptomyces sp. |
3.2.1.52 | Triton X-100 | loss of 70% of activity with 0.2% after 5 h at 37°C | Streptomyces sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.52 | Streptomyces sp. | - |
S. plicatus | - |
3.2.1.52 | Streptomyces sp. | - |
ATCC 27800 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.52 | to homogeneity | Streptomyces sp. |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.2.1.52 | (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | endo-type reaction | Streptomyces sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.52 | culture filtrate | - |
Streptomyces sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 0.97 | - |
pool III | Streptomyces sp. |
3.2.1.52 | 1 | - |
pool I | Streptomyces sp. |
3.2.1.52 | 1.05 | - |
pool II | Streptomyces sp. |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.52 | 4°C, crude supernatant fraction stable for at least a year | Streptomyces sp. |
3.2.1.52 | 4°C, purified enzyme after sterilization stable for at least 6 months | Streptomyces sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.52 | (GlcNAc)2Man + H2O | - |
Streptomyces sp. | ? | - |
? | |
3.2.1.52 | (N-acetyl-beta-D-glucosamine)3 + H2O | - |
Streptomyces sp. | (N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine | - |
? | |
3.2.1.52 | Asn(GlcNAc)2Man + H2O | - |
Streptomyces sp. | ? | - |
? | |
3.2.1.52 | chitin oligosaccharides + H2O | (N-acetyl-D-glucosamine)2-6 | Streptomyces sp. | N-acetyl-D-glucosamine | - |
? | |
3.2.1.52 | dansyl-Asn(GlcNAc)2 + H2O | - |
Streptomyces sp. | ? | - |
? | |
3.2.1.52 | dansyl-Asn(GlcNAc)2Man + H2O | - |
Streptomyces sp. | dansyl-AsnGlcNAc + ? | - |
? | |
3.2.1.52 | dansyl-Asn(GlcNAc)2Man2 + H2O | - |
Streptomyces sp. | ? | - |
? | |
3.2.1.52 | GlcNAC-beta-(1->4)-GlcNAC-beta-(1->4)-GlcNAC-beta-(1->4)-GlcNAc-ol + H2O | - |
Streptomyces sp. | ? | - |
? | |
3.2.1.52 | N,N',N'',N'''-tetraacetylchitotetraose + H2O | - |
Streptomyces sp. | 2 N,N-diacetylchitobiose | - |
? | |
3.2.1.52 | N,N',N''-triacetylchitotriose + H2O | - |
Streptomyces sp. | N-acetyl-D-glucosamine + N,N'-diacetylchitobiose | - |
? | |
3.2.1.52 | N,N'-diacetylchitobiose + H2O | - |
Streptomyces sp. | deacetylated chitooligosaccharides + acetate | - |
? | |
3.2.1.52 | N-acetyl-beta-D-hexosaminides + H2O | - |
Streptomyces sp. | N-acetyl-D-hexosamines | - |
? | |
3.2.1.52 | p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Streptomyces sp. | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.52 | endo-beta-N-acetylglucosaminidase | - |
- |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | - |
- |
pH from 3.0-8.0, stable for 24 h | Streptomyces sp. |
3.2.1.52 | 25 | - |
pH from 3.0-8.0, stable for 24 h | Streptomyces sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 4 | 4.5 | substrate: dansyl-Asn(GlcNAc)2Man | Streptomyces sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 5.5 | - |
half-maximal activity | Streptomyces sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 3.5 | - |
half-maximal activity | Streptomyces sp. |