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Literature summary extracted from

  • Dimond, R.L.; Loomis, W.F.
    Vegetative isozyme of N-acetylglucosaminidase in Dictyostelium discoideum (1974), J. Biol. Chem., 249, 5628-5632.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Ag+ minor isozyme: 2% of activity remains with 5 mM, major isozyme: 38% of activity remains with 2 mM Dictyostelium discoideum
3.2.1.52 Cu2+ minor isozyme: 19% of activity remains with 10 mM, major isozyme: 47% of activity remains with 10 mM Dictyostelium discoideum
3.2.1.52 Hg2+ minor isozyme: 5% of activity remains with 0.01 mM, major isozyme: 53% of activity remains with 0.01 mM Dictyostelium discoideum
3.2.1.52 Zn2+ minor isozyme: 8% of activity remains with 5 mM, major isozyme: 40% of activity remains with 2 mM Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 1.5
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Dictyostelium discoideum
3.2.1.52 1.5
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside extract of wild type strain, purified major and minor isozyme Dictyostelium discoideum
3.2.1.52 7.8
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside extract of mutant strains Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Dictyostelium discoideum
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Dictyostelium discoideum
-
-
-
3.2.1.52 Dictyostelium discoideum A3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 stage-specific enzyme: 2200fold, vegetative enzyme: 1400fold Dictyostelium discoideum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 1.4
-
 vegetative enzyme Dictyostelium discoideum
3.2.1.52 262
-
 stage-specific enzyme Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 chitin + H2O
-
 Dictyostelium discoideum N-acetylglucosamine
-
 ?
3.2.1.52 chitin + H2O
-
 Dictyostelium discoideum A3 N-acetylglucosamine
-
 ?
3.2.1.52 chondroitinsulfate C + H2O
-
 Dictyostelium discoideum ?
-
 ?
3.2.1.52 hyaluronic acid + H2O
-
 Dictyostelium discoideum N-acetylglucosamine + ?
-
 ?
3.2.1.52 hyaluronic acid + H2O
-
 Dictyostelium discoideum A3 N-acetylglucosamine + ?
-
 ?
3.2.1.52 murein + H2O bacterial cell wall murein, activity against this substrate at pH 3.5 and not 5.0 Dictyostelium discoideum ?
-
 ?
3.2.1.52 murein + H2O bacterial cell wall murein, activity against this substrate at pH 3.5 and not 5.0 Dictyostelium discoideum A3 ?
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Dictyostelium discoideum N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Dictyostelium discoideum 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Dictyostelium discoideum A3 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Dictyostelium discoideum 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Dictyostelium discoideum A3 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 55
-
 major peak: half-life of 17 min at pH 5.0, minor peak: half-life of more than 100 min at pH 5.0 Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 3.5
-
 extract of mutant strains Dictyostelium discoideum
3.2.1.52 5
-
 extract of wild type strain Dictyostelium discoideum