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Literature summary extracted from

  • Koga, D.; Shimazaki, C.; Yamamoto, K.; Inoue, K.; Kimura, S.; Ide, A.
    beta-N-Acetyl-D-glucosaminidases from integument of the silkworm, Bombyx mori: Comparative biochemistry with the pupal alimentary canal enzyme (1987), Agric. Biol. Chem., 51, 1679-1681.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Hg2+ Ex 1, Ex 2 and alimentary canal enzyme Bombyx mori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.03
-
 (N-acetyl-beta-D-glucosamine)4 Ex 2 Bombyx mori
3.2.1.52 0.05
-
 (N-acetyl-beta-D-glucosamine)3 Ex 2 Bombyx mori
3.2.1.52 0.06
-
 (N-acetyl-beta-D-glucosamine)6 Ex 2 Bombyx mori
3.2.1.52 0.07
-
 (N-acetyl-beta-D-glucosamine)5 Ex 1 Bombyx mori
3.2.1.52 0.09
-
 (N-acetyl-beta-D-glucosamine)5 Ex 2 Bombyx mori
3.2.1.52 0.1
-
 (N-acetyl-beta-D-glucosamine)6 Ex 1 Bombyx mori
3.2.1.52 0.16
-
 (N-acetyl-beta-D-glucosamine)4 Ex 1 Bombyx mori
3.2.1.52 0.31
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Ex 2 Bombyx mori
3.2.1.52 0.42
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Ex 2 Bombyx mori
3.2.1.52 0.49
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Ex 1 Bombyx mori
3.2.1.52 0.64
-
 (N-acetyl-beta-D-glucosamine)2 Ex 2 Bombyx mori
3.2.1.52 0.64
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Ex 1 Bombyx mori
3.2.1.52 0.67
-
 (N-acetyl-beta-D-glucosamine)2 Ex 1 Bombyx mori
3.2.1.52 0.87
-
 (N-acetyl-beta-D-glucosamine)3 Ex 1 Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 57500
-
 alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE Bombyx mori
3.2.1.52 57500
-
 Ex 2: 1 * 67500 + 1 * 57500, SDS-PAGE Bombyx mori
3.2.1.52 67500
-
 alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE Bombyx mori
3.2.1.52 67500
-
 Ex 2: 1 * 67500 + 1 * 57500, SDS-PAGE Bombyx mori
3.2.1.52 67500
-
 Ex 1, homodimer: 2 * 67500 Bombyx mori
3.2.1.52 125000
-
 Ex 2 and alimentary canal enzyme, gel filtration Bombyx mori
3.2.1.52 135000
-
 Ex 1, gel filtration Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Bombyx mori
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Bombyx mori
-
 silkworm, pupae and fifth-instar larvae at the SP3 stage
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 Ex 1: 3000fold, Ex 2: 3200fold Bombyx mori

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose exo-type reaction Bombyx mori
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 alimentary canal
-
 Bombyx mori
-
3.2.1.52 integument
-
 Bombyx mori
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 (N-acetyl-beta-D-glucosamine)2 + H2O
-
 Bombyx mori 2 N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 (N-acetyl-beta-D-glucosamine)3 + H2O
-
 Bombyx mori (N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 (N-acetyl-beta-D-glucosamine)4 + H2O
-
 Bombyx mori (N-acetyl-beta-D-glucosamine)3 + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 (N-acetyl-beta-D-glucosamine)5 + H2O
-
 Bombyx mori (N-acetyl-beta-D-glucosamine)4 + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 (N-acetyl-beta-D-glucosamine)6 + H2O
-
 Bombyx mori (N-acetyl-beta-D-glucosamine)5 + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 chitin oligosaccharides + H2O (N-acetyl-D-glucosamine)2-6 Bombyx mori N-acetyl-D-glucosamine
-
 ?
3.2.1.52 N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Bombyx mori ?
-
 ?
3.2.1.52 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Bombyx mori ?
-
 ?
3.2.1.52 N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Bombyx mori 2 N,N-diacetylchitobiose
-
 ?
3.2.1.52 N,N',N''-triacetylchitotriose + H2O
-
 Bombyx mori N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
 Bombyx mori deacetylated chitooligosaccharides + acetate
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Bombyx mori N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Bombyx mori 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Bombyx mori 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 dimer alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE Bombyx mori
3.2.1.52 dimer Ex 2: 1 * 67500 + 1 * 57500, SDS-PAGE Bombyx mori
3.2.1.52 dimer Ex 1, homodimer: 2 * 67500 Bombyx mori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 45
-
 alimentary canal enzyme Bombyx mori
3.2.1.52 55
-
 Ex 1 Bombyx mori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 40
-
 alimentary canal enzyme, stable below Bombyx mori
3.2.1.52 48
-
 Ex 1, stable below Bombyx mori
3.2.1.52 58
-
 alimentary canal enzyme, 50% of activity remains Bombyx mori
3.2.1.52 62
-
 Ex 1, 50% of activity remains Bombyx mori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.52 29
-
 (N-acetyl-beta-D-glucosamine)6 Ex 2 Bombyx mori
3.2.1.52 42
-
 (N-acetyl-beta-D-glucosamine)4 Ex 2 Bombyx mori
3.2.1.52 55
-
 (N-acetyl-beta-D-glucosamine)6 Ex 1 Bombyx mori
3.2.1.52 57
-
 (N-acetyl-beta-D-glucosamine)5 Ex 1 Bombyx mori
3.2.1.52 76
-
 (N-acetyl-beta-D-glucosamine)5 Ex 2 Bombyx mori
3.2.1.52 82
-
 (N-acetyl-beta-D-glucosamine)3 Ex 2 Bombyx mori
3.2.1.52 110
-
 (N-acetyl-beta-D-glucosamine)4 Ex 1 Bombyx mori
3.2.1.52 130
-
 (N-acetyl-beta-D-glucosamine)3 Ex 1 Bombyx mori
3.2.1.52 320
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Ex 2 Bombyx mori
3.2.1.52 570
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Ex 1 Bombyx mori
3.2.1.52 580
-
 (N-acetyl-beta-D-glucosamine)2 Ex 2 Bombyx mori
3.2.1.52 620
-
 (N-acetyl-beta-D-glucosamine)2 Ex 1 Bombyx mori
3.2.1.52 720
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Ex 2 Bombyx mori
3.2.1.52 890
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Ex 1 Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 5 6 Ex 1, Ex 2 and alimentary canal enzyme Bombyx mori

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 5 9 Ex 2, stable Bombyx mori
3.2.1.52 5 8.5 alimentary canal enzyme, stable Bombyx mori
3.2.1.52 6 8 Ex 1, stable Bombyx mori