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Literature summary extracted from

  • Sakai, K.; Narihara, M.; Kasama, Y.; Wakayama, M.; Moriguchi, M.
    Purification and characterization of thermostable beta-N-acetylhexosaminidase of Bacillus stearothermophilus CH-4 isolated from chitin-containing compost (1994), Appl. Environ. Microbiol., 60, 2911-2915.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 Ca2+ concentration of 4.0 mM: activity reaches 171% Geobacillus stearothermophilus
3.2.1.52 D-galactosamine concentration of 100 mM: activity reaches 140% Geobacillus stearothermophilus
3.2.1.52 D-glucosamine concentration of 30 mM: activity reaches 140% Geobacillus stearothermophilus
3.2.1.52 Fe2+ concentration of 0.6 mM: activity reaches 204% Geobacillus stearothermophilus
3.2.1.52 Zn2+ concentration of 0.4 mM: activity reaches 142% Geobacillus stearothermophilus

General Stability

EC Number General Stability Organism
3.2.1.52 at 70°C for 10 min: most stable in potassium phosphate buffer, more stable under alkaline than acidic conditions Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 chitobiose concentration of 10 mM causes 25% inhibition Geobacillus stearothermophilus
3.2.1.52 D-mannose concentration of 10 mM causes 33% inhibition Geobacillus stearothermophilus
3.2.1.52 fructose concentration of 10 mM causes 38% inhibition Geobacillus stearothermophilus
3.2.1.52 galactose concentration of 10 mM causes 15% inhibition Geobacillus stearothermophilus
3.2.1.52 glucose concentration of 10 mM causes 30% inhibition Geobacillus stearothermophilus
3.2.1.52 Hg2+
-
 Geobacillus stearothermophilus
3.2.1.52 lactose concentration of 10 mM causes 25% inhibition Geobacillus stearothermophilus
3.2.1.52 Mn2+ slight inhibition Geobacillus stearothermophilus
3.2.1.52 N-acetyl-D-galactosamine concentration of 10 mM causes 28% inhibition Geobacillus stearothermophilus
3.2.1.52 N-acetyl-D-glucosamine competitive inhibitor; concentration of 10 mM causes 80% inhibition Geobacillus stearothermophilus
3.2.1.52 sucrose concentration of 10 mM causes 9% inhibition Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.13
-
 4-nitrophenyl N,N'-diacetylchitobioside
-
 Geobacillus stearothermophilus
3.2.1.52 0.17
-
 4-nitrophenyl N,N',N''-triacetylchitotrioside
-
 Geobacillus stearothermophilus
3.2.1.52 0.28
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Geobacillus stearothermophilus
3.2.1.52 0.57
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Geobacillus stearothermophilus
3.2.1.52 0.59
-
 p-nitrophenyl-thio-beta-N-acetyl-D-glucosaminide
-
 Geobacillus stearothermophilus
3.2.1.52 4.1
-
 (N-acetyl-beta-D-glucosamine)2
-
 Geobacillus stearothermophilus
3.2.1.52 8
-
 (N-acetyl-beta-D-glucosamine)3
-
 Geobacillus stearothermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 extracellular
-
 Geobacillus stearothermophilus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 74000
-
 SDS-PAGE Geobacillus stearothermophilus
3.2.1.52 75000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Geobacillus stearothermophilus
-
 N-acetyl-D-hexosamines
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.52 acetonitrile
-
 Geobacillus stearothermophilus
3.2.1.52 dimethyl sulfoxide 80%, 18.0% of activity remains Geobacillus stearothermophilus
3.2.1.52 dimethylformamide 80%, 13.9% of activity remains Geobacillus stearothermophilus
3.2.1.52 dioxane
-
 Geobacillus stearothermophilus
3.2.1.52 Ethanol
-
 Geobacillus stearothermophilus
3.2.1.52 guanidine-HCl 4 M at 37°C for 30 min, 54% of activity remains Geobacillus stearothermophilus
3.2.1.52 n-Butanol
-
 Geobacillus stearothermophilus
3.2.1.52 n-propanol
-
 Geobacillus stearothermophilus
3.2.1.52 Pyridine
-
 Geobacillus stearothermophilus
3.2.1.52 sodium dodecyl sulfate 0.1% and 1% SDS decrease activity to 66% and 26% Geobacillus stearothermophilus
3.2.1.52 urea 8 M at 37°C for 30 min, 91% of activity remains Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Geobacillus stearothermophilus
-
 CH-4
-
3.2.1.52 Geobacillus stearothermophilus CH-4
-
 CH-4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 387fold to homogeneity Geobacillus stearothermophilus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 culture filtrate
-
 Geobacillus stearothermophilus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 11.7
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 (N-acetyl-beta-D-glucosamine)2 + H2O
-
 Geobacillus stearothermophilus 2 N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 (N-acetyl-beta-D-glucosamine)3 + H2O
-
 Geobacillus stearothermophilus (N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl N,N',N''-triacetylchitotrioside + H2O
-
 Geobacillus stearothermophilus 4-nitrophenol + N,N',N''-triacetylchitotriose
-
 ?
3.2.1.52 4-nitrophenyl N,N',N''-triacetylchitotrioside + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
3.2.1.52 4-nitrophenyl N,N'-diacetylchitobioside + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
3.2.1.52 4-nitrophenyl N,N'-diacetylchitobioside + H2O
-
 Geobacillus stearothermophilus 4-nitrophenol + N,N'-diacetylchitobiose
-
 ?
3.2.1.52 N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
3.2.1.52 N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Geobacillus stearothermophilus CH-4 ?
-
 ?
3.2.1.52 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
3.2.1.52 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Geobacillus stearothermophilus CH-4 ?
-
 ?
3.2.1.52 N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Geobacillus stearothermophilus 2 N,N-diacetylchitobiose
-
 ?
3.2.1.52 N,N',N''-triacetylchitotriose + H2O
-
 Geobacillus stearothermophilus N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
 Geobacillus stearothermophilus deacetylated chitooligosaccharides + acetate
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
 Geobacillus stearothermophilus CH-4 deacetylated chitooligosaccharides + acetate
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Geobacillus stearothermophilus N-acetyl-D-hexosamines
-
 ?
3.2.1.52 N-acetyllactosamine + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Geobacillus stearothermophilus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Geobacillus stearothermophilus CH-4 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Geobacillus stearothermophilus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Geobacillus stearothermophilus CH-4 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-thio-beta-N-acetyl-D-glucosaminide + H2O
-
 Geobacillus stearothermophilus 4-nitrophenol + thio-beta-N-acetyl-D-glucosaminide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 monomer 1 * 74000 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 75
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 60
-
 stable for 10 min Geobacillus stearothermophilus
3.2.1.52 80
-
 retains 28% of activity after 10 min Geobacillus stearothermophilus
3.2.1.52 90
-
 complete inactivation after 10 min Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 6.5
-
-
 Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 6.5
-
 stable Geobacillus stearothermophilus