Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chang, C.T.; Young, F.P.; Chang, M.H.; Sung, H.Y.
    Purification and properties of beta-N-acetylhexosaminidase from cabbage (1998), Biochem. Mol. Biol. Int., 45, 371-380.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 diethyl dicarbonate with 30 mM 1.1% of activity remains Brassica oleracea
3.2.1.52 N-bromosuccinimide with 0.025 mM 4.4% of activity remains Brassica oleracea
3.2.1.52 N-ethyl-5-phenyl-isoxazolium-3'-sulfonate with 30 mM 23.3% of activity remains Brassica oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.94
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Brassica oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 51000
-
 1 * 64000 + 1 * 57000 + 1 * 51000, SDS-PAGE Brassica oleracea
3.2.1.52 57000
-
 1 * 64000 + 1 * 57000 + 1 * 51000, SDS-PAGE Brassica oleracea
3.2.1.52 64000
-
 1 * 64000 + 1 * 57000 + 1 * 51000, SDS-PAGE Brassica oleracea
3.2.1.52 150000
-
 gel filtration Brassica oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Brassica oleracea
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Brassica oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 256fold to homogeneity Brassica oleracea

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose exo-type reaction Brassica oleracea
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 leaf
-
 Brassica oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 9.737
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Brassica oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 N,N',N'',N'''-tetraacetylchitotetraose + 3 H2O
-
 Brassica oleracea 4 N-acetyl-D-glucosamine
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
 Brassica oleracea N-acetyl-D-glucosamine
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Brassica oleracea N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Brassica oleracea 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Brassica oleracea 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 tri-N-acetylchitotriose + H2O
-
 Brassica oleracea N-acetyl-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 trimer 1 * 64000 + 1 * 57000 + 1 * 51000, SDS-PAGE Brassica oleracea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 60
-
-
 Brassica oleracea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 30
-
 stable below for 15 min at pH 7.0 Brassica oleracea
3.2.1.52 35 60 for 15 min at pH 7.0, gradual inactivation Brassica oleracea
3.2.1.52 65
-
 for 15 min at pH 7.0, rapid inactivation Brassica oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Brassica oleracea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 3 9 stable for 4 hr at room temperature Brassica oleracea
3.2.1.52 10
-
 rapidly inactivated above, at room temperature Brassica oleracea