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Literature summary extracted from

  • Keyhani, N.O.; Roseman, S.
    The chitin catabolic cascade in the marine bacterium Vibrio furnissii: Molecular cloning, isolation, and characterization of a periplasmic beta-N-acetylhexosaminidase (1996), J. Biol. Chem., 271, 33425-33432.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52 into Escherichia coli Vibrio furnissii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 (N-acetyl-D-glucosamine)2 non-competitive Vibrio furnissii
3.2.1.52 (N-acetyl-D-glucosamine)3-6 competitive inhibition Vibrio furnissii
3.2.1.52 cellobiose
-
Vibrio furnissii
3.2.1.52 Hg2+ with 1 mM 50% of activity remains, with 5 mM 10% of activity remains Vibrio furnissii
3.2.1.52 KCl with 1 mM 80% of activity remains, with 2 mM 50% of activity remains Vibrio furnissii
3.2.1.52 lactose
-
Vibrio furnissii
3.2.1.52 maltose
-
Vibrio furnissii
3.2.1.52 additional information
-
Vibrio furnissii
3.2.1.52 NaCl with 1 mM 80% of activity remains, with 2 mM 50% of activity remains Vibrio furnissii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.09
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
Vibrio furnissii
3.2.1.52 0.33
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
Vibrio furnissii
3.2.1.52 0.78
-
(N-acetyl-D-glucosamine)4
-
Vibrio furnissii
3.2.1.52 0.83
-
(N-acetyl-D-glucosamine)3
-
Vibrio furnissii
3.2.1.52 1.7
-
(N-acetyl-D-glucosamine)2
-
Vibrio furnissii
3.2.1.52 3.5
-
(N-acetyl-D-glucosamine)6
-
Vibrio furnissii
3.2.1.52 3.7
-
(N-acetyl-D-glucosamine)5
-
Vibrio furnissii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 periplasm
-
Vibrio furnissii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 69380
-
amino acid calculation Vibrio furnissii
3.2.1.52 70000
-
SDS-PAGE Vibrio furnissii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Vibrio furnissii
-
N-acetyl-D-hexosamines
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Vibrio furnissii P96155
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 40fold to homogeneity Vibrio furnissii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 200
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Vibrio furnissii

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 -70°C, purified enzyme, stable for at least 6 months Vibrio furnissii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
Vibrio furnissii 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
?
3.2.1.52 aryl-beta-N-acetylgalactosaminide + H2O
-
Vibrio furnissii a phenol + beta-N-acetylgalactosamine
-
?
3.2.1.52 aryl-beta-N-acetylglucosaminide + H2O
-
Vibrio furnissii ? + beta-N-acetyl-D-glucosamine
-
?
3.2.1.52 chitin oligosaccharides + H2O (N-acetyl-D-glucosamine)2-6 Vibrio furnissii N-acetyl-D-glucosamine
-
?
3.2.1.52 N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
Vibrio furnissii ?
-
?
3.2.1.52 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
Vibrio furnissii ?
-
?
3.2.1.52 N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
Vibrio furnissii 2 N,N-diacetylchitobiose
-
?
3.2.1.52 N,N',N''-triacetylchitotriose + H2O
-
Vibrio furnissii N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
?
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
Vibrio furnissii deacetylated chitooligosaccharides + acetate
-
?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
Vibrio furnissii N-acetyl-D-hexosamines
-
?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
Vibrio furnissii 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
Vibrio furnissii 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 35 40 at pH 7.5, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Vibrio furnissii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 4 42 stable for 15 min, at pH 7.5 Vibrio furnissii
3.2.1.52 20
-
stable for at least 24 h at room temperature Vibrio furnissii
3.2.1.52 37
-
stable for at least 24 h Vibrio furnissii
3.2.1.52 65
-
complete loss of activity after 15 min at pH 7.5 Vibrio furnissii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 5.8
-
substrate: (N-acetyl-D-glucosamine)2 Vibrio furnissii
3.2.1.52 7 8 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Vibrio furnissii
3.2.1.52 7
-
substrate: (N-acetyl-D-glucosamine)3-6 Vibrio furnissii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 5.5 9.5 full activity at pH 7.5 after preincubation for 15 min in that range, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Vibrio furnissii
3.2.1.52 6
-
50% of activity, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Vibrio furnissii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 7.5
-
no activity, substrate: (N-acetyl-D-glucosamine)2 Vibrio furnissii
3.2.1.52 8 8.3 50-60% of activity with (N-acetyl-D-glucosamine)3-6 as substrate, no activity with (N-acetyl-D-glucosamine)2 as substrate Vibrio furnissii
3.2.1.52 9
-
50% of activity, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Vibrio furnissii