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Literature summary extracted from

  • Bouquelet, S.; Spik, G.
    Properties of four molecular forms of N-acetyl-beta-D-hexosaminidase isolated from germinating seeds of fenugreek (Trigonella foenum graecum) (1978), Eur. J. Biochem., 84, 551-559.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 bovine serum albumin at 5% both hexosaminidase activities of forms II and I are increased 2- and 1.5fold Trigonella foenum-graecum
3.2.1.52 Fe2+ stimulates both hexosaminidase activities of form I Trigonella foenum-graecum
3.2.1.52 Fe3+ stimulates galactosaminidase activitiy of form I Trigonella foenum-graecum
3.2.1.52 L-cysteine concentrations of 0.05, 0.1, 0.4 and 0.7 mM are needed to reactivate both hexosaminidase activities of forms I, II, III and IV Trigonella foenum-graecum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Ag+ all 4 forms Trigonella foenum-graecum
3.2.1.52 Fe2+ both hexosaminidase activities of forms II, III and IV inhibited Trigonella foenum-graecum
3.2.1.52 Fe3+ glucosaminidase-activity of forms II, III and IV are depressed, galactosaminidase-activity of forms III and IV are inhibited Trigonella foenum-graecum
3.2.1.52 Hg2+ all 4 forms Trigonella foenum-graecum
3.2.1.52 p-chloromercuribenzoate inhibits forms II, III and IV, competitive inhibition Trigonella foenum-graecum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 additional information
-
 additional information KM 0.056, new form I, after 4 months of storage at 4°C Trigonella foenum-graecum
3.2.1.52 0.04
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form I Trigonella foenum-graecum
3.2.1.52 0.04
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside form IV Trigonella foenum-graecum
3.2.1.52 0.087
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form III Trigonella foenum-graecum
3.2.1.52 0.09
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside form III Trigonella foenum-graecum
3.2.1.52 0.15
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside form I Trigonella foenum-graecum
3.2.1.52 0.37
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form IV Trigonella foenum-graecum
3.2.1.52 0.55
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside form II Trigonella foenum-graecum
3.2.1.52 0.62
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form II Trigonella foenum-graecum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 24000
-
 form II, SDS-PAGE Trigonella foenum-graecum
3.2.1.52 28000
-
 form I, SDS-PAGE Trigonella foenum-graecum
3.2.1.52 30000
-
 form IV, SDS-PAGE Trigonella foenum-graecum
3.2.1.52 30500
-
 form III, SDS-PAGE Trigonella foenum-graecum
3.2.1.52 62000
-
 new form I, after 4 months of storage at 4°C Trigonella foenum-graecum
3.2.1.52 72000
-
 form II, gel filtration Trigonella foenum-graecum
3.2.1.52 84000
-
 form I, gel filtration Trigonella foenum-graecum
3.2.1.52 150000
-
 form IV, gel filtration Trigonella foenum-graecum
3.2.1.52 180000
-
 form III, gel filtration Trigonella foenum-graecum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Trigonella foenum-graecum
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Trigonella foenum-graecum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.52 glycoprotein mannosylation Trigonella foenum-graecum
3.2.1.52 glycoprotein N-acetyl-glucosaminylation Trigonella foenum-graecum
3.2.1.52 glycoprotein fucosylation Trigonella foenum-graecum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 form I: 2262fold, form II: 2102fold, form III: 20380fold, form IV: 5061fold Trigonella foenum-graecum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 cotyledon forms I and II Trigonella foenum-graecum
-
3.2.1.52 embryo forms III and IV Trigonella foenum-graecum
-
3.2.1.52 endosperm forms I and II Trigonella foenum-graecum
-
3.2.1.52 seed germinating seeds Trigonella foenum-graecum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 2.64
-
 form II Trigonella foenum-graecum
3.2.1.52 2.82
-
 form I Trigonella foenum-graecum
3.2.1.52 6.35
-
 form IV Trigonella foenum-graecum
3.2.1.52 25.68
-
 form III Trigonella foenum-graecum

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 4°C, after 2 months of storage in phosphate buffer pH 5.0 only form I shows decrease of activity by 50-60%, after 4 months a new form of enzyme with a molecular weight of 62000 Da and a Km of 0.056 mM appears Trigonella foenum-graecum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O
-
 Trigonella foenum-graecum 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-galactopyranoside
-
 ?
3.2.1.52 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Trigonella foenum-graecum 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 ?
3.2.1.52 additional information
-
 Trigonella foenum-graecum ?
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Trigonella foenum-graecum N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Trigonella foenum-graecum 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Trigonella foenum-graecum 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 hexamer 6 * 30500, SDS-PAGE, form III Trigonella foenum-graecum
3.2.1.52 pentamer 5 * 30000, SDS-PAGE, form IV Trigonella foenum-graecum
3.2.1.52 trimer 3 * 28000, SDS-PAGE, form I Trigonella foenum-graecum
3.2.1.52 trimer 3 * 24000, SDS-PAGE, form II Trigonella foenum-graecum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 40 45 all 4 forms Trigonella foenum-graecum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 37
-
 at its optimum pH both hexosaminidase activities of each of the 4 forms remain stable over 5 hours Trigonella foenum-graecum
3.2.1.52 50
-
 both hexosaminidase activities of form I decrease rapidly, forms II, III and IV show biphasic denaturation curves Trigonella foenum-graecum
3.2.1.52 60
-
 complete inactivation of all 4 forms after 20 min Trigonella foenum-graecum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4.4
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, form III Trigonella foenum-graecum
3.2.1.52 4.6
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, form IV Trigonella foenum-graecum
3.2.1.52 4.8
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form IV Trigonella foenum-graecum
3.2.1.52 5
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, forms II and III Trigonella foenum-graecum
3.2.1.52 5
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, form II Trigonella foenum-graecum
3.2.1.52 5.5
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, form I Trigonella foenum-graecum
3.2.1.52 6
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form I Trigonella foenum-graecum