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Literature summary extracted from

  • Sarber, R.L.; Distler, J.J.; Jourdian, G.W.
    beta-N-Acetylhexosaminidase from bovine testes (1978), Methods Enzymol., 50, 520-523.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.52 no loss of activity on repeated freezing and thawing Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 EDTA with 50 mM 54% inhibition Bos taurus
3.2.1.52 Sodium acetate with 50 mM 27% inhibition Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 61000
-
 SDS-PAGE Bos taurus
3.2.1.52 62500
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Bos taurus
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 testis
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 -20°C, stable for at least 1 year Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 glycopeptides derived from asialo ovalbumin + H2O hydrolyzes terminal and nonreducing residues, no hydrolysis of the intact glycoprotein Bos taurus ?
-
 ?
3.2.1.52 glycopeptides derived from asialo ovomucoid + H2O hydrolyzes terminal and nonreducing residues, no hydrolysis of the intact glycoprotein Bos taurus ?
-
 ?
3.2.1.52 methyl-beta-N-acetyl-glucosaminide + H2O
-
 Bos taurus methanol + beta-N-acetyl-glucosamine
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
 Bos taurus N-acetyl-D-glucosamine
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Bos taurus N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Bos taurus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Bos taurus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 tri-N-acetylchitotriose + H2O
-
 Bos taurus N-acetyl-D-glucosamine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 37
-
 in presence of 1% bovine serum albumin and 0.1% sodium azide at pH 4.5 stable for at least 72 h Bos taurus