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Literature summary extracted from

  • Farooqui, A.A.; Srivastava, P.N.
    Isolation of beta-N-acetylhexosaminidase from rabbit semen and its role in fertilization (1980), Biochem. J., 191, 827-834.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 glutathione 1 mM leads to moderate stimulation of p-chloromercuribenzoate-treated enzyme, no effect alone Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Ag+ 1 and 2 mM cause 70 and 92% inhibition respectively Oryctolagus cuniculus
3.2.1.52 Hg2+ 0.05 and 0.1 mM cause 45 and 70% inhibition Oryctolagus cuniculus
3.2.1.52 p-chloromercuribenzoate 0.025 and 0.05 mM cause 70 and 83% inhibition Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 1.4
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 cytoplasm
-
 Oryctolagus cuniculus 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Oryctolagus cuniculus
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 226fold to homogeneity Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 semen
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 56
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Oryctolagus cuniculus N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Oryctolagus cuniculus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 55
-
 purified enzyme loses 85% of activity after 20 min Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Oryctolagus cuniculus