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Literature summary extracted from

  • Uda, Y.; Itoh, T.
    Purification and characterization of beta-N-acetylhexosaminidase from the ascidian, Halocynthia roretzi (1983), J. Biochem., 93, 847-855.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Ag+ with 0.1 mM 63% of activity remains Halocynthia roretzi
3.2.1.52 Cu2+ with 10 mM 47% of activity remains Halocynthia roretzi
3.2.1.52 Hg2+ concentration of 0.1 mM results in total loss of activity Halocynthia roretzi
3.2.1.52 sodium taurodeoxycholate
-
 Halocynthia roretzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.87
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Halocynthia roretzi
3.2.1.52 1.92
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Halocynthia roretzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 74000
-
 SDS-PAGE Halocynthia roretzi
3.2.1.52 170000
-
 at pH 7.0, gel filtration Halocynthia roretzi
3.2.1.52 330000
-
 at pH 4.5, gel filtration Halocynthia roretzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Halocynthia roretzi
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Halocynthia roretzi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 820fold Halocynthia roretzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 viscus
-
 Halocynthia roretzi
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 214.1
-
-
 Halocynthia roretzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 asialo GM2 ganglioside + H2O
-
 Halocynthia roretzi lactosylceramide
-
 ?
3.2.1.52 globoside I + H2O
-
 Halocynthia roretzi trihexaosylceramide + GalNAc
-
 ?
3.2.1.52 GM2 ganglioside + H2O
-
 Halocynthia roretzi GM3 ganglioside + N-acetyl-beta-D-galactosamine
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Halocynthia roretzi N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Halocynthia roretzi 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Halocynthia roretzi 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 40
-
 stable for 30 min Halocynthia roretzi
3.2.1.52 50
-
 loses 30% of activity after 30 min Halocynthia roretzi
3.2.1.52 60
-
 loses almost all activity after 5 min Halocynthia roretzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Halocynthia roretzi
3.2.1.52 4.2
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Halocynthia roretzi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 4.5
-
 active Halocynthia roretzi

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 4 7.5 stable Halocynthia roretzi
3.2.1.52 7
-
 inactive Halocynthia roretzi