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Literature summary extracted from
- Purification and characterization of beta-N-acetylhexosaminidase from Penicillium oxalicum (1985), Agric. Biol. Chem., 49, 611-619.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.52 | Hg2+ | 0.05 mM results in significant inhibition | Penicillium oxalicum |  |
| 3.2.1.52 | p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | at higher concentrations | Penicillium oxalicum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.52 | 0.48 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Penicillium oxalicum | |
| 3.2.1.52 | 1 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | - |
Penicillium oxalicum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.52 | extracellular | - |
Penicillium oxalicum | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.52 | 68000 | - |
2 * 68000, SDS-PAGE | Penicillium oxalicum |
| 3.2.1.52 | 141000 | - |
sedimentation equilibrium | Penicillium oxalicum |
| 3.2.1.52 | 143000 | - |
gel filtration | Penicillium oxalicum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.52 | N-acetyl-beta-D-hexosaminides + H2O | Penicillium oxalicum | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.52 | Penicillium oxalicum | - |
IFO 5748 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.52 | glycoprotein | - |
Penicillium oxalicum |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.52 | to homogeneity | Penicillium oxalicum |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.2.1.52 | (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | exo-type reaction | Penicillium oxalicum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.52 | culture filtrate | - |
Penicillium oxalicum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.52 | 126 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Penicillium oxalicum |
| 3.2.1.52 | 186 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | Penicillium oxalicum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.52 | -20°C, enzyme preparation concentrated by ultrafiltration | Penicillium oxalicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.52 | glycopeptide + H2O | glycopeptide obtained by modification of fetuin | Penicillium oxalicum | N-acetyl-D-glucosamine | - |
? | |
| 3.2.1.52 | N,N'-diacetylchitobiose + H2O | - |
Penicillium oxalicum | N-acetyl-D-glucosamine | - |
? | |
| 3.2.1.52 | N-acetyl-beta-D-hexosaminides + H2O | - |
Penicillium oxalicum | N-acetyl-D-hexosamines | - |
? | |
| 3.2.1.52 | p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Penicillium oxalicum | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
| 3.2.1.52 | p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Penicillium oxalicum | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
| 3.2.1.52 | tri-N-acetylchitotriose + H2O | - |
Penicillium oxalicum | N-acetyl-D-glucosamine | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.52 | dimer | 2 * 68000, SDS-PAGE | Penicillium oxalicum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.52 | 55 | - |
- |
Penicillium oxalicum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.52 | 45 | - |
stable for 10 min | Penicillium oxalicum |
| 3.2.1.52 | 65 | - |
complete inactivation within 10 min | Penicillium oxalicum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.52 | 3 | 4.5 | - |
Penicillium oxalicum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.52 | 2.5 | 5 | active within this range | Penicillium oxalicum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.52 | 7 | 9 | stable within this range | Penicillium oxalicum |
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