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Literature summary extracted from

  • Poulton, J.E.; Thomas, M.A.; Ottwell, K.K.; McCormick, S.J.
    Partial purification and characterization of a beta-N-acetylhexosaminidase from black cherry (Prunus serotina Ehrh.) seeds (1985), Plant Sci., 42, 107-114.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.52 purification by N-acetylglucosamine-agarose columns results in extreme unstability of enzyme, extensive purification requires bovine serum albumin for retention of activity Prunus serotina

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Fe2+ 1 mM reduces activity by 59% Prunus serotina
3.2.1.52 Fe3+ concentration of 10 mM reduces activity by 23% Prunus serotina
3.2.1.52 Hg2+ 1 mM reduces activity by 89% Prunus serotina
3.2.1.52 N-acetyl-D-galactosamine 45% inhibition at 20 mM Prunus serotina
3.2.1.52 p-chloromercuriphenylsulfonate concentration of 10 mM inhibited activity by 46% Prunus serotina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.49
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Prunus serotina
3.2.1.52 0.63
-
 4-nitrophenyl N-acetyl-beta-D-galactosaminide
-
 Prunus serotina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Prunus serotina
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Prunus serotina
-
 black cherry
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 partial purification, 30fold, more extensive purification requires bovine serum albumin for retention of activity Prunus serotina

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose same active site for N-glucosaminidase and N-galactosaminidase activities Prunus serotina
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 184.2
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Prunus serotina

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 4°C, enzyme purified in presence of bovine serum albumin, storage in presence of 0.04% w/v sodium azide, enzyme retains 76% of activity after 5 months Prunus serotina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-nitrophenyl beta-D-galactopyranoside + H2O at 3.6% of the rate observed with p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Prunus serotina 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Prunus serotina 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Prunus serotina N-acetyl-D-hexosamines
-
 ?
3.2.1.52 o-nitrophenyl-beta-D-glucoside + H2O at 4.2% of the rate observed with p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Prunus serotina 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Prunus serotina 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Prunus serotina 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-D-glucopyranoside + H2O at 17% of the rate observed with p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Prunus serotina 4-nitrophenol + beta-D-glucose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Prunus serotina
3.2.1.52 4.5
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Prunus serotina