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Literature summary extracted from

  • Watanabe, Y.; Moriyama, R.; Matsuda, T.; Shimizu, S.; Ohmiya, K.
    Purification and properties of the endo-1,4-beta-glucanase III from Ruminococcus albus (1992), J. Ferment. Bioeng., 73, 54-57.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 2-mercaptoethanol slight activation Ruminococcus albus
3.2.1.4 Cysteine hydrochloride slight activation Ruminococcus albus
3.2.1.4 dithiothreitol slight activation Ruminococcus albus
3.2.1.4 glutathione slight activation Ruminococcus albus

General Stability

EC Number General Stability Organism
3.2.1.4 2-mercaptoethanol, dithiothreitol, glutathione and cysteine-HCl stabilize Ruminococcus albus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Cu2+
-
 Ruminococcus albus
3.2.1.4 Fe2+
-
 Ruminococcus albus
3.2.1.4 Hg2+
-
 Ruminococcus albus
3.2.1.4 iodoacetamide
-
 Ruminococcus albus
3.2.1.4 NEM
-
 Ruminococcus albus
3.2.1.4 PCMB
-
 Ruminococcus albus
3.2.1.4 Zn2+
-
 Ruminococcus albus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 0.72
-
 cellohexaose
-
 Ruminococcus albus
3.2.1.4 7.24
-
 cellopentaose
-
 Ruminococcus albus
3.2.1.4 8.85
-
 cellotetraose
-
 Ruminococcus albus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 53000
-
 gel filtration Ruminococcus albus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Ruminococcus albus
-
 endoglucanase III
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 endoglucanase III Ruminococcus albus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 additional information
-
-
 Ruminococcus albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethylcellulose + H2O
-
 Ruminococcus albus additional information
-
 ?
3.2.1.4 cellohexaose + H2O
-
 Ruminococcus albus additional information
-
 ?
3.2.1.4 cellopentaose + H2O
-
 Ruminococcus albus additional information
-
 ?
3.2.1.4 cellotetraose + H2O
-
 Ruminococcus albus 2 cellobiose
-
 ?
3.2.1.4 cellotriose + H2O negligibly hydrolyzed Ruminococcus albus D-glucose + cellobiose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 55
-
 carboxymethylcellulose-degrading activity Ruminococcus albus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5.7
-
 carboxymethylcellulose-degrading activity Ruminococcus albus