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Literature summary extracted from

  • Wilson, D.B.
    Cellulases of Thermomonospora fusca (1988), Methods Enzymol., 160, 314-323.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Ag+ AgNO3, endoglucanase E1 and E2 Thermobifida fusca
3.2.1.4 Hg2+ endoglucanase E1 and E2; HgCl2 Thermobifida fusca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information
-
 Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Thermobifida fusca
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 glycoprotein enzyme E2 and E3 are glycoproteins Thermobifida fusca
3.2.1.4 no modification enzyme E1, E4 and E5 are not glycoproteins Thermobifida fusca

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 enzyme form E1, E2, E3, E4 and E5 Thermobifida fusca

Storage Stability

EC Number Storage Stability Organism
3.2.1.4 -70°C, purified enzymes are very stable Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethylcellulose + H2O
-
 Thermobifida fusca additional information
-
 ?
3.2.1.4 cellotriose + H2O hydrolyzed by endoglucanase E1, no activity with endoglucanase E2 Thermobifida fusca D-glucose + cellobiose + glucose, endoglucanase E1 ?
3.2.1.4 cellulose + H2O amorphous cellulose Thermobifida fusca ? endoglucanase E1 produces mainly cellobiose and a trace of glucose, endoglucanase E2 produces mainly cellotriose and higher oligomers with some cellobiose and a trace of glucose ?
3.2.1.4 xylan + H2O
-
 Thermobifida fusca additional information
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6.5
-
 endoglucanase E1 and E2 Thermobifida fusca