Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • De la Cruz, J.; Hidalgo-Gallego, A.; Lora, J.M.; Benitez, T.; Pintor-Toro, J.A.; Llobell, A.
    Isolation and characterization of three chitinases from Trichoderma harzianum (1992), Eur. J. Biochem., 206, 859-867.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 additional information colloidal chitin, chit33 0.3 mg/ml, chit37 0.5 mg/ml, chit42 1 mg/ml Trichoderma harzianum
3.2.1.14 0.7
-
 p-nitrophenyl beta-D-N,N'-diacetylchitobiose chit37 Trichoderma harzianum
3.2.1.14 0.85
-
 p-nitrophenyl beta-D-N,N'-diacetylchitobiose chit42 Trichoderma harzianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Trichoderma harzianum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 33000
-
 chit33, gel filtration and SDS-PAGE Trichoderma harzianum
3.2.1.14 37000
-
 chit37, gel filtration and SDS-PAGE Trichoderma harzianum
3.2.1.14 42000
-
 chit42, gel filtration and SDS-PAGE Trichoderma harzianum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Trichoderma harzianum
-
 CECT 2413
-
3.2.1.14 Trichoderma harzianum CECT 2413
-
 CECT 2413
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 chit33 1.3fold to homogeneity, chit42 8fold to homogeneity, chit37 3.4fold to homogeneity Trichoderma harzianum

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.2.1.14
-
 Trichoderma harzianum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 culture filtrate
-
 Trichoderma harzianum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 1.1
-
 chit33 Trichoderma harzianum
3.2.1.14 2.78
-
 chit37 Trichoderma harzianum
3.2.1.14 6.54
-
 chit42 Trichoderma harzianum

Storage Stability

EC Number Storage Stability Organism
3.2.1.14 4°C, stable for at least one month Trichoderma harzianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O chit37 and chit42 Trichoderma harzianum 4-methylumbelliferone + N,N'-diacetylchitobiose
-
 ?
3.2.1.14 cell wall + H2O chit42, cell wall from Botrytis cinerea Trichoderma harzianum chitin oligosaccharides
-
 ?
3.2.1.14 chitin + H2O colloidal chitin Trichoderma harzianum N,N-diacetylchitobiose + N-acetylglucosamine chit37 and chit42 main product chitobiose, some N-acetylglucosamine and chitotriose also, chit33 main product chitotetraose, chitobiose, chitotetraose, n-acetylglucosamine also present ?
3.2.1.14 chitin + H2O colloidal chitin Trichoderma harzianum CECT 2413 N,N-diacetylchitobiose + N-acetylglucosamine chit37 and chit42 main product chitobiose, some N-acetylglucosamine and chitotriose also, chit33 main product chitotetraose, chitobiose, chitotetraose, n-acetylglucosamine also present ?
3.2.1.14 chitopentaose + H2O
-
 Trichoderma harzianum chitobiose + chitotriose main product chitobiose, small amounts of chitotriose and N-acetylglucosamine also ?
3.2.1.14 chitopentaose + H2O
-
 Trichoderma harzianum CECT 2413 chitobiose + chitotriose main product chitobiose, small amounts of chitotriose and N-acetylglucosamine also ?
3.2.1.14 glycol chitin + H2O
-
 Trichoderma harzianum N-acetylglucosamine + ?
-
 ?
3.2.1.14 glycol chitin + H2O
-
 Trichoderma harzianum CECT 2413 N-acetylglucosamine + ?
-
 ?
3.2.1.14 glycol chitosan + H2O chit37 and chit42 Trichoderma harzianum ?
-
 ?
3.2.1.14 glycol chitosan + H2O chit37 and chit42 Trichoderma harzianum CECT 2413 ?
-
 ?
3.2.1.14 N,N',N''-triacetylchitotriose + H2O
-
 Trichoderma harzianum N-acetylglucosamine + N,N'-diacetylchitobiose
-
 ?
3.2.1.14 N,N',N''-triacetylchitotriose + H2O
-
 Trichoderma harzianum CECT 2413 N-acetylglucosamine + N,N'-diacetylchitobiose
-
 ?
3.2.1.14 p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
-
 Trichoderma harzianum p-nitrophenol + N,N'-diacetylchitobiose
-
 ?
3.2.1.14 p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O chit37 and chit42 Trichoderma harzianum N,N'-diacetylchitobiose + p-nitrophenol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 40 45 chit42 Trichoderma harzianum
3.2.1.14 45 50 chit33 and chit37 Trichoderma harzianum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 50
-
 stable below 50°C, chit42 Trichoderma harzianum
3.2.1.14 60
-
 stable below 60°C, chit33 and chit37 Trichoderma harzianum