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Literature summary extracted from

  • Molano, J.; Polacheck, I.; Duran, A.; Cabib, E.
    An endochitinase from wheat germ. Activity on nascent and preformed chitin (1979), J. Biol. Chem., 254, 4901-4907.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 diacetylchitobiose competitive inhibitor Triticum aestivum
3.2.1.14 N-acetylglucosamine poor competitor Triticum aestivum
3.2.1.14 pentaacetylchitopentaose competitive inhibitor Triticum aestivum
3.2.1.14 tetraacetylchitotetraose competitive inhibitor Triticum aestivum
3.2.1.14 triacetylchitotriose competitive inhibitor Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 2
-
 chitin chitin concentration calculated as N-acetylglucosamine Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 29000
-
 gel filtration Triticum aestivum
3.2.1.14 30000
-
 SDS-PAGE Triticum aestivum
3.2.1.14 33000
-
 sedimentation equilibrium Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Triticum aestivum
-
 wheat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 to homogeneity, 300fold Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 germ
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 14.6
-
 calculated as N-acetylglucosamine Triticum aestivum

Storage Stability

EC Number Storage Stability Organism
3.2.1.14 -20°C, 0.02% azide, several weeks, no loss of activity Triticum aestivum
3.2.1.14 2°C, 0.02% azide, several weeks, no loss of activity Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O [acetyl-3H]chitin Triticum aestivum N,N-diacetylchitobiose + N-acetylglucosamine chitobiose, biotriose and biotetraose from nascent chitin, higher oligosaccharides from preformed chitin ?
3.2.1.14 chitohexaose + H2O
-
 Triticum aestivum chitobiose + chitotriose
-
 ?
3.2.1.14 chitopentaose + H2O
-
 Triticum aestivum chitobiose + chitotriose
-
 ?
3.2.1.14 chitosan + H2O
-
 Triticum aestivum ?
-
 ?
3.2.1.14 chitotetraose + H2O tritiated Triticum aestivum N-acetylglucosamine + chitobiose + chitotriose only traces of chitotriose and N-acetylglucosamine ?
3.2.1.14 glycol chitin + H2O tritiated and unlabeled Triticum aestivum N-acetylglucosamine + ?
-
 ?
3.2.1.14 N,N',N''-triacetylchitotriose + H2O 3H labelled, only slightly attacked Triticum aestivum N-acetylglucosamine + N,N'-diacetylchitobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 monomer 1 * 30000, SDS-PAGE Triticum aestivum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 30
-
-
 Triticum aestivum
3.2.1.14 56
-
-
 Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 6
-
-
 Triticum aestivum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 3
-
 75% activity Triticum aestivum
3.2.1.14 9
-
 53% activity Triticum aestivum