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Literature summary extracted from
- Serratia marcescens chitinase: one-step purification and use for the determination of chitin (1982), Anal. Biochem., 127, 402-412.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.14 | 4.4 | - |
chitin | expressed in N-acetylglucosamine equivalents | Serratia marcescens |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.14 | extracellular | - |
Serratia marcescens | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 52000 | - |
x * 52000 + x * 58000, SDS-PAGE | Serratia marcescens |
| 3.2.1.14 | 58000 | - |
x * 52000 + x * 58000, SDS-PAGE | Serratia marcescens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | Serratia marcescens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | 5.9fold | Serratia marcescens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | culture filtrate | - |
Serratia marcescens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 1.33 | - |
- |
Serratia marcescens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | cell wall + H2O | from yeast | Serratia marcescens | chitin oligosaccharides | N-acetetylglucosamine and diacetylchitobiose | ? | |
| 3.2.1.14 | chitin + H2O | from ground crab shell | Serratia marcescens | N,N-diacetylchitobiose + N-acetylglucosamine | with high ratio of chitinase to chitin N-acetylglucosamine only | ? | |
| 3.2.1.14 | chitin + H2O | [acetyl-3H]chitin | Serratia marcescens | N,N-diacetylchitobiose + N-acetylglucosamine | with high ratio of chitinase to chitin N-acetylglucosamine only | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | ? | x * 52000 + x * 58000, SDS-PAGE | Serratia marcescens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 4 | 7 | - |
Serratia marcescens |
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