Any feedback?
Literature summary extracted from
- Chitinase and beta-N-acetylhexosaminidase from Pycnoporus cinnabarinus (1988), Methods Enzymol., 161, 462-470.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.52 | relatively stable to freezing and thawing in 0.05 M citrate buffer pH 4.5 | Trametes cinnabarina |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.52 | Hg2+ | slight inhibition at 10 mM | Trametes cinnabarina |  |
| 3.2.1.52 | additional information | not: N-acetyl-D-galactosamine | Trametes cinnabarina | |
| 3.2.1.52 | N,N,N,N-tetraacetylchitotetraose | inhibits hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside stronger than hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Trametes cinnabarina | |
| 3.2.1.52 | N,N,N-triacetylchitotriose | inhibits hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside stronger than hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Trametes cinnabarina | |
| 3.2.1.52 | N,N-diacetylchitobiose | inhibits hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside stronger than hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Trametes cinnabarina | |
| 3.2.1.52 | N-acetyl-D-glucosamine | competitive inhibitor | Trametes cinnabarina | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.52 | 0.45 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Trametes cinnabarina | |
| 3.2.1.52 | 0.54 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | - |
Trametes cinnabarina | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.14 | extracellular | - |
Trametes cinnabarina | - |
- |
| 3.2.1.52 | extracellular | - |
Trametes cinnabarina | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 38000 | - |
gel filtration | Trametes cinnabarina |
| 3.2.1.52 | 65000 | - |
SDS-PAGE | Trametes cinnabarina |
| 3.2.1.52 | 120000 | - |
gel filtration | Trametes cinnabarina |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.52 | N-acetyl-beta-D-hexosaminides + H2O | Trametes cinnabarina | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | Trametes cinnabarina | - |
- |
- |
| 3.2.1.52 | Trametes cinnabarina | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | 14.8fold | Trametes cinnabarina |
| 3.2.1.52 | 1400fold | Trametes cinnabarina |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.2.1.52 | (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | exo-type reaction | Trametes cinnabarina |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | culture filtrate | - |
Trametes cinnabarina | - |
| 3.2.1.52 | culture filtrate | - |
Trametes cinnabarina | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 6.22 | - |
- |
Trametes cinnabarina |
| 3.2.1.52 | 67 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Trametes cinnabarina |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | chitin + H2O | colloidal and glycol chitin, powdered chitin very slowly | Trametes cinnabarina | N,N-diacetylchitobiose + N-acetylglucosamine | - |
? | |
| 3.2.1.14 | chitooligosaccharides + H2O | higher than trisaccharides, also reduced chitooligosaccharides more than tetrasaccharides | Trametes cinnabarina | ? | - |
? | |
| 3.2.1.14 | N,N',N''-triacetylchitotriose + H2O | - |
Trametes cinnabarina | N,N'-diacetylchitobiose + N-acetylglucosamine | - |
? | |
| 3.2.1.52 | N,N'-diacetylchitobiose + H2O | - |
Trametes cinnabarina | N-acetyl-D-glucosamine | - |
? | |
| 3.2.1.52 | N-acetyl-beta-D-hexosaminides + H2O | - |
Trametes cinnabarina | N-acetyl-D-hexosamines | - |
? | |
| 3.2.1.52 | p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Trametes cinnabarina | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
| 3.2.1.52 | p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Trametes cinnabarina | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
| 3.2.1.52 | reduced chitooligosaccharides + H2O | - |
Trametes cinnabarina | N-acetylglucosamine | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | additional information | - |
unstable at elevated temperatures | Trametes cinnabarina |
| 3.2.1.52 | 5 | - |
stable at this temperature between pH 2.0-4.0 | Trametes cinnabarina |
| 3.2.1.52 | 50 | - |
unstable above | Trametes cinnabarina |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 4.5 | - |
- |
Trametes cinnabarina |
| 3.2.1.52 | 2.5 | - |
substrate: N,N-diacetylchitobiose and p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Trametes cinnabarina |
| 3.2.1.52 | 3.7 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | Trametes cinnabarina |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 4 | 8 | - |
Trametes cinnabarina |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
