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Literature summary extracted from

  • Essigmann, B.; Hespenheide, B.M.; Kuhn, L.A.; Benning, C.
    Prediction of the active-site structure and NAD+ binding in SQD1, a protein essential for sulfolipid biosynthesis in Arabidopsis (1999), Arch. Biochem. Biophys., 369, 30-41.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.1.1 expressed in Escherichia coli Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.13.1.1 chloroplast
-
 Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.1.1 UDP-glucose + ? Arabidopsis thaliana first step of sulfolipid biosynthesis, unknown sulfur donor UDP-sulfoquinovose + H2O
-
 ?
3.13.1.1 UDP-glucose + sulfite Arabidopsis thaliana
-
 UDP-sulfoquinovose + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.13.1.1 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.1.1 Ni2+-nitrilotriacetic acid column Arabidopsis thaliana

Reaction

EC Number Reaction Comment Organism Reaction ID
3.13.1.1 UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite sequence similarity to sugar nucleotide modifying enzymes, Thr228 seems to be catalytically important Arabidopsis thaliana
a
3.13.1.1 UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite short-chain dehydrogenase/reductase family Arabidopsis thaliana
a

Storage Stability

EC Number Storage Stability Organism
3.13.1.1 4°C, 50 mM MES buffer, pH 6.5, 10 mM UDP-glucose, stable for 10 days Arabidopsis thaliana
3.13.1.1 4°C, 50 mM MES buffer, pH 6.5, stable for 2-3 days Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.1.1 UDP-glucose + ? first step of sulfolipid biosynthesis, unknown sulfur donor Arabidopsis thaliana UDP-sulfoquinovose + H2O
-
 ?
3.13.1.1 UDP-glucose + sulfite
-
 Arabidopsis thaliana UDP-sulfoquinovose + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
3.13.1.1 NAD+ tightly bound, enzyme binds 1 NAD+ per monomer, side-chain chemistry and hydrogen-bonding patterns in the NAD+ site Arabidopsis thaliana